Author:
Zhang Guanghai,Yao Heng,Zhao Gaokun,Wu Yuping,Xia Huachan,Li Yongping,Kong Guanghui
Abstract
Abstract
Background
The quality of cigar tobacco leaves was closely related to fermentation. To investigate the substance changes in cigar tobacco leaves during their fermentation, metabolomics was determined at different fermentation stages. In this study, the metabolic profile among different regions and fermentation periods of cigar tobacco leaf were evaluated.
Results
A total of 1103 metabolites were identified in cigar tobacco leaf samples. A total of 293, 105 and 199 metabolites showed differential accumulation in the cigar tobacco leaf among different regions (PEF0 vs. LCF0, PEF0 vs. DHF0, PEF0 vs. YXF0) and 216, 242, 220, 227 and 198 metabolites showed differential accumulation in the different fermentation (LCF0 vs. LCF1, LCF0 vs. LCF2, LCF0 vs. LCF3, LCF0 vs. LCF4, LCF0 vs. LCF5). The main upregulated compounds were flavonoids, phenolic acids and lignans and coumarins, and the main downregulated compounds were organic acids, phenolic acids and amino acids and derivatives in the fermentation comparison group.
Conclusions
These results provide valuable information for accurately grasping the end time of fermentation and improve efficiency of cigar tobacco leaf fermentation.
Graphical Abstract
Funder
Yunnan Provincial Tobacco Monopoly Bureau Grants
the China Tobacco Monopoly Bureau Grants and Yunnan Provincial Tobacco Monopoly Bureau Grants
Publisher
Springer Science and Business Media LLC
Subject
Agronomy and Crop Science,Biochemistry,Food Science,Biotechnology
Reference23 articles.
1. Frankenburg WG. Chemical changes in the harvested tobacco leaf. II chemical and enzymic conversions during fermentation and aging. In: Nord FF, editor. Advances in enzymology and related areas of molecular biology: nord/advances. Hoboken: John Wiley & Sons, Inc.; 1950.
2. Frankenburg WG. Chemical changes in the harvested tobacco leaf: part I chemical and enzymic conversions during the curing process. In: Nord FF, editor. Advances in enzymology and related areas of molecular biology: nord/advances. Hoboken: John Wiley & Sons, Inc.; 1946.
3. Wen Z, Liu Y, Zeng D, Yang J, Xi H. The study and application of cigar filler stacking fermentation technology. Hans J Agric Sci. 2016;06:63–70. https://doi.org/10.1267/HJAS.2016.63010.
4. Liu F, Wu Z, Zhang X, Xi G, Zhao Z, Lai M, Zhao M. Microbial community and metabolic function analysis of cigar tobacco leaves during fermentation. MicrobiologyOpen. 2021. https://doi.org/10.1002/mbo3.1171.
5. Dai J, Dong A, Xiong G, Liu Y, Hossain MS, Liu S, Gao N, Li S, Wang J, Qiu D. Production of highly active extracellular amylase and cellulase from bacillus subtilis ZIM3 and a recombinant strain with a potential application in tobacco fermentation. Front Microbiol. 2020;11:1539. https://doi.org/10.3389/fmicb.2020.01539.
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