Abstract
Abstract
Background
Nowadays Alzheimer’s disease and its treatment methods are global concerns. Patients with this disease have poor prognosis and need supportive treatment. The antioxidant activity, anti-acetylcholinesterase (anti-AChE), anti-butyryl cholinesterase (anti-BChE) and Aβ-amyloid-42 inhibition activities of the ethanolic extracts of both leaves and flowers (LEE and FEE) of Markhamia lutea were assessed. The antioxidant activity of LEE and FEE was evaluated using 2,2-diphenyl-1-picryl-hydrazyl-hydrate, oxygen radical absorbance capacity and ferrozine iron metal chelation assays. Additionally, their total flavonoids and total phenolics were determined. The phytochemicals of LEE were analysed using LC–MS/Q-TOF in both positive and negative modes. Also, molecular docking was done for phytochemicals identified in LEE.
Result
LEE exhibited higher antioxidant and anti-Alzheimer activities in all techniques due to its high flavonoids content. LEE showed better activity than donepezil in case of anti-butyryl cholinesterase than both donepezil and rivastigmine in case of Aβ-amyloid-42 inhibition. A total of 62 compounds were tentatively identified using Ultra-performance Liquid Chromatography-Electrospray Ionization-Quadrupole Time-of-Flight Mass Spectrometry (UHPLC–ESI–TOF–MS), viz. 35 flavonoids, 11 phenolic acids, 2 terpenoids, 2 phenylpropanoids derivatives, 7 polyphenols, 3 coumarins and 2 organic acids. The molecular docking of some constituents showed that isorhamnetin-O-rutinoside, sissotrin, 3,5,7-trihydroxy-4'-methoxyflavone (diosmetin), rosmarinic acid, kaempferol hexoside, kampferol-7-neohesperoside, acacetin, taxifolin and apigenin-O-hexoside exert a promising activity as anti-Alzheimer drugs.
Conclusion
The LEE of Markhamia lutea contains secondary metabolites that is promising to act as natural antioxidants, acetylcholinesterase, butyryl cholinesterase and Aβ-amyloid-42 inhibition inhibitors, which can aid in the treatment of Alzheimer’s.
Graphical Abstract
Publisher
Springer Science and Business Media LLC
Reference40 articles.
1. de Almeida RB et al (2023) Identification of a novel dual inhibitor of acetylcholinesterase and butyrylcholinesterase: in vitro and in silico studies. Pharmaceuticals 16(1):95
2. Adetuyi AR, Ayenero ME, Olaleye MT, Akindahunsi AA, Akinmoladun AC (2024) Antioxidant and acetylcholinesterase inhibitory activities, in silico analyses, and anti-Alzheimer’s disease potential of leaf extracts of three Nigerian endemic medicinal plants (Spondias mombin, Carica pa paya and Kalanchoe crenata). FJPS 10(1):6
3. Althobaiti NA, Menaa F, Dalzell JJ, Albalawi AE, Ismail H, Alghuthaymi MA, Aldawsari RD, Iqbal H, McAlinney C, Green BD (2022) Ethnomedicinal plants with protective effects against beta-amyloid peptide (Aβ) 1–42 indicate therapeutic potential in a new in vivo model of Alzheimer’s disease. Antioxidants 11(10):1865
4. Yeniçeri E, Altay A, Koksal E, Altın S, Taslimi P, Yılmaz MA, Cakir O, Tarhan A, Kandemir A (2024) Phytochemical profile by LC–MS/MS analysis and evaluation of antioxidant, antidiabetic, anti-Alzheimer, and anticancer activity of Onobrychis argyrea leaf extracts. Eur J Integr Med 66:102337
5. Fathallah N, El Deeb M, Rabea AA, Almehmady AM, Alkharobi H, Elhady SS, Khalil NJM (2023) Ultra-performance liquid chromatography coupled with mass metabolic profiling of Ammi majus roots as waste product with isolation and assessment of oral mucosal toxicity of its psoralen component Xanthotoxin. Mol 13(10):1044