Author:
Md Toha Zaleha,Haron Nor Hasyimah,Kamal Nik Nur Syazni Nik Mohamed,Khairuddean Melati,Arsad Hasni
Abstract
Abstract
Background
Medicinal herbs in Malaysia like Clinacanthus nutans (CN) traditionally are used in the treatment of various diseases and cancers. The present research was conducted to determine the effects of C. nutans leaf different solvent extracts on the human breast cancer cell lines (MCF-7). The antiproliferative growth and survival effects of dichloromethane CN leaf extracts (CNDCM), as well as the short- and long-term effects through metastasis, apoptosis and cell cycle effects, were observed. The chemical profiles were done to evaluate the properties of the CNDCM.
Results
The evaluation of GC–MS identified 16 major phytochemical compounds present in this extract with biological activities. Antiproliferative assay used is the SRB assay, which showed the CNDCM induced strong antiproliferative property compared with the other extracts, so its IC50 dose was selected for further testing with value 108 µg/mL at 72 h after exposure on MCF-7 and MCF-10A cell lines. The clonogenic survival effects of CNDCM in various concentrations (31.25, 62.5, 125, 250 and 500 µg/mL) inhibited the ability of MCF-7 cells to form colonies, and the metastasis result was indicated in an image of wound healing assay. Moreover, the CNDCM extract significantly induced apoptosis in all the cell cycle phases. Finally, the experiments with various extract concentrations on normal human breast cell lines showed no antiproliferative effects for all the extracts tested.
Conclusion
Among all the extracts of CN, the CNDCM extracts demonstrated the highest antiproliferative activity and survival against the MCF-7 cell lines tested.
Funder
FRGS grant
University of Southern Maine
Publisher
Springer Science and Business Media LLC
Reference45 articles.
1. Boontha S, Thoedyotin T, Saengtabtim T, Im-erb P, Chaniad N, Buranrat B, Pitaksuteepong T (2020) Cytotoxic, colony formation and anti-migratory effects of Spilanthes acmella (Asteraceas) aerial extract on MCF-7 cells and its cream formulation. Trop J Pharm Res 19(1):17–24
2. Md Toha Z, Zainoddin HN, Aziz WNW, Arsad H (2018) Antiproliferative effect of the methanol extract of Goniothalamus umbrosus leaves on HeLa cells. J Biol Sci Opin 6(2):17–22
3. Amaral KC, de Carvalho L, Albiero AL, Laube R, Nascimento ACG, Bonfilm L, Vieira DP (2019) Effects of an inhibitor of nitric oxide production on cell cycle and micronucleus frequently in irradiated human breast adenocarcinoma cells. In: International nuclear Atlantic conference—INAC 2019
4. Pelegrina LT, de los Angeles Sanhueza M, Caceres AR, Cuello-Carrion D, Rodriguez CE, Laconi MR (2020) Effect of progesterone and first evidence about allopregnanolone action on the progression of epithelial human ovarian cancer cell lines. J Steroid Biochem Mol Biol 196:105492
5. Chamcheu JC, Rady I, Chamcheu R-CN, Siddique AB, Bloch MB, Mbeumi SB, Babatundae AS, Uddin MB, Noubissi FK, Jurukat PW, Liu YY, Spiegelman VS, Whitfield GK, El-Sayed Ka (2018) Graviola (Annona muricata ) exerts antiproliferative, anti-clonogenic and pro-apoptosis effects in human non-melanoma skin cancer UW-BCC1 and A431 cells in vitro: involvement of Hegehog Signaling. Int J Mol Sci 19(6):1–24
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