Author:
Gupta Praveen Suresh,Patel Shivani
Abstract
AbstractBackgroundPlants have been used in alternative and traditional medicines for the cure of different types of diseases since ancient time. Secondary metabolites from natural sources play a crucial role in the treatment of various ailments. The present study carried out to investigate the phytochemical, antimitotic and cytotoxic activity of methanolic (95%) extracts ofMucuna pruriensseeds,Asteracantha longifoliaseeds andSphaeranthus indicusstems.ResultPhytochemical analysis was performed using qualitative test to confirm the presence of phytochemical such as flavonoids, terpenoids, amino acids, cardiac glycosides, saponins, steroids, tannins, phenols and carbohydrates. The antimitotic activity was screened by usingAllium ceparoot meristematic cells. Methotrexate (0.1 mg/mL) was used as a standard. The data was analyzed by using software GraphPad Prism, Version 6.0 (GraphPad Software Inc., San Diego, CA) with one-way ANOVA. A statistical difference ofp< 0.05 was considered significant in all cases.pvalueof M. pruriensseeds,A. longifoliaseeds andS. indicusstems calculatedp= 0.0001 for all plant extracts. Cytotoxic potential of all three plant extracts have been studied on breast cancer cell line MCF7 and lung cancer cell line A549.M. pruriensshowed mild cytotoxicity with IC50values 36.74 μg/mL on MCF7 and 39.42 μg/mL on A549 cell line.A. longifoliashowed better activity on MCF7 with IC50of 12.32 μg/mL and theS. indicusshowed the least activity on MCF7 with IC50of 185.56 μg/mL. TheA. longifoliashowed better activity on A549 with IC50of 16.53 μg/mL.ConclusionA. longifoliahas significant amount of nearly all phytochemicals as compared to other two plant extracts. It is found that all three plant extracts showed antimitotic activity havingpvalue less than 0.05. The cytotoxicity assay revealed that all plant extracts displayed inhibition of MCF7 and A549 cells lines.A. longifoliashowed better activity against MCF7 whileM. prurienspossessed mild cytotoxic effect against both MCF7 and A549 cell lines.
Publisher
Springer Science and Business Media LLC
Reference43 articles.
1. Sharma S, Kaushik R, Sharma P, Sharma R, Thapa A, Indumathi KP (2016) Antimicrobial activity of herbs against Yersinia enterocolitica and mixed microflora. The Annals of the University Dunarea de Jos of Galati. Food Technol 40(2):119–134
2. Aggarwal BB, Kumar A, Bharti AC (2003) Anticancer potential of curcumin: Preclinical and clinical studies. Anticancer Res 23(1A):363–398
3. Veeresham C (2012) Natural products derived from plants as a source of drugs. J Adv Pharm Technol Res 3(4):200–201. https://doi.org/10.4103/2231-4040.104709
4. Cragg GM, Newman DJ (2005) Plants as a source of anti-cancer agents. J Ethnopharmacol 100(1-2):72–79. https://doi.org/10.1016/j.jep.2005.05.011
5. Bray F, Ferlay J, Soerjomataram I, Siegel RL, Torre LA, Jemal A (2018) Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA: Cancer J Clin 68(6):394–424. https://doi.org/10.3322/caac.21492
Cited by
6 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献