Author:
Jones Mary,Helliwell Peter,Pritchard Colin,Tharakan Joseph,Mathew Joseph
Abstract
Abstract
Background
This pilot study was carried out to determine whether Helicobacter pylori can be detected in normal colon or in association with colorectal neoplasia.
Methods
Paraffin processed colonic tissue blocks of normal colonic mucosa (n = 60), and patients diagnosed as adenoma (n = 60), and adenocarcinoma (n = 60) were retrieved from our archive; the adenoma group included tubular (n = 20), tubulovillous (n = 20) and villous adenomas (n = 20). 4 μm sections were stained by immunohistochemical methods using anti-Helicobacter pylori antibodies (polyclonal NCL-HPp and monoclonal NCL-C-jejuni).
Results
Significant numbers of Helicobacter pylori were identified in tubular adenomas (OR = 11.13; 95%CI = 1.62–76.70), tubulovillous adenomas (OR = 10.45; 95%CI = 1.52–71.52) and adenocarcinomas (OR = 8.13; 95%CI = 1.40–46.99) compared to controls: there was no association in numbers of Helicobacter pylori and villous adenomas (OR = 2.95; 95%CI = 0.29–9.96).
Conclusion
We conclude that although, in this pilot study, there appears to be an association in the prevalence of Helicobacter pylori with some, but not all, colorectal neoplasms, we can not infer causality from these results. These findings need to be further substantiated with a prospective study and the use of molecular biological techniques to determine a causal association.
Publisher
Springer Science and Business Media LLC
Reference54 articles.
1. Cummings OW: Pathology of the adenoma-carcinoma sequence: from aberrant crypt focus to invasive carcinoma. Semin Gastrointest Dis. 2000, 11: 229-237.
2. Hardy RG, Meltzer SJ, Jankowski JA: ABC of colorectal cancer: Molecular basis for risk factors. BMJ. 2000, 321: 886-889. 10.1136/bmj.321.7265.886.
3. Day DW, Jass JR, Price AB, Shepherd NA, Sloan JM, Talbot IC, Warren BF, Williams GT: Morson and Dawson's gastrointestinal pathology. 2003, Oxford, Blackwell Science Ltd, 4th
4. Gryfe R, Swallow C, Bapat B, Redston M, Gallinger S, Couture J: Molecular biology of colorectal cancer. Curr Probl Cancer. 1997, 21: 233-300. 10.1016/S0147-0272(97)80003-7.
5. Liu C, Crawford JM: The gastrointestinal tract. Robbins and Cotran Pathologic Basis of Disease. Edited by: Kumar V, Abbas AK and Fausto N. 2005, Philadelphia, Elsevier Saunders, 797-875. 7th
Cited by
62 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献