Author:
Khan Mohammed I.,Czarnecka Anna M.,Helbrecht Igor,Bartnik Ewa,Lian Fei,Szczylik Cezary
Abstract
AbstractIn recent years, cancer stem cells (CSCs)/tumor initiating cells (TICs) have been identified inside different tumors. However, currently used anti-cancer therapies are mostly directed against somatic tumor cells without targeting CSCs/TICs. CSCs/TICs also gain resistance to chemotherapies/radiotherapies. For the development of efficient treatment strategies, choosing the best method for isolation and characterization of CSCs/TICs is still debated among the scientific community. In this review, we summarize recent data concerning isolation techniques for CSCs using magnetic cell sorting and flow cytometry. The review focuses on the strategies for sample preparation during flow cytometric analysis, elaborating biomarkers such as CXCR4, CD105, and CD133. In addition, functional properties characteristic of CSCs/TICs using side population selection through Hoechst 33342 dye, aldehyde dehydrogenase 1, dye-cycle violet, and rhodamine 123 are also discussed. We also include a special focus on enriching CSCs/TICs using three-dimensional cell culture models such as agarose–agarose microbeads and sphere formation.
Publisher
Springer Science and Business Media LLC
Subject
Cell Biology,Biochemistry, Genetics and Molecular Biology (miscellaneous),Molecular Medicine,Medicine (miscellaneous)
Reference84 articles.
1. Duff SE, Li C, Garland JM, Kumar S. CD105 is important for angiogenesis: evidence and potential applications. FASEB J. 2003;17:984–92.
2. Bussolati B, Bruno S, Grange C, Ferrando U, Camussi G. Identification of a tumor-initiating stem cell population in human renal carcinomas. FASEB J. 2008;22:3696–705.
3. Khan MI, Czarnecka AM, Szczylik C. Detection of CD105+ and CD133+ sub-populations (cancer initiating cells) in SMKT-R2, SMKT-R3 and 786-O human primary renal cancer cell lines [Abstract]. In: Wiersdorff V, editor. The 4th EMBO meeting; September 22–25, 2012. France.: Nice; 2012. p. 212–3.
4. Khan MI, Czarnecka AM, Krol M, Zdanowski R, Sobocinska A, Lewicki S, et al. Analysis of tumor initiating cells (TICs) populations in primary and metastatic cell lines from clear cell renal cell carcinoma (ccRCC) [Abstract]. Mumbai: 4th International Conference on Stem Cells and Cancer (ICSCC-2013); 2013 October 19–22; 2013. p. 73–4.
5. Li Z. CD133: a stem cell biomarker and beyond. Exp Hematol Oncol. 2013;2:17.
Cited by
54 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献