Author:
Tian Chuan,He Jie,An Yuanyuan,Yang Zailing,Yan Donghai,Pan Hang,Lv Guanke,Li Ye,Wang Yanying,Yang Yukun,Zhu Gaohong,He Zhixu,Zhu Xiangqing,Pan Xinghua
Abstract
Abstract
Background
Female sex hormone secretion and reproductive ability decrease with ageing. Bone marrow mesenchymal stem cells (BMMSCs) have been postulated to play a key role in treating ovarian ageing.
Methods
We used macaque ovarian ageing models to observe the structural and functional changes after juvenile BMMSC treatment. Moreover, RNA-seq was used to analyse the ovarian transcriptional expression profile and key pathways through which BMMSCs reverse ovarian ageing.
Results
In the elderly macaque models, the ovaries were atrophied, the regulation ability of sex hormones was reduced, the ovarian structure was destroyed, and only local atretic follicles were observed, in contrast with young rhesus monkeys. Intravenous infusion of BMMSCs in elderly macaques increased ovarian volume, strengthened the regulation ability of sex hormones, reduced the degree of pulmonary fibrosis, inhibited apoptosis, increased density of blood vessels, and promoted follicular regeneration. In addition, the ovarian expression characteristics of ageing-related genes of the elderly treatment group reverted to that of the young control group, 1258 genes that were differentially expressed, among which 415 genes upregulated with age were downregulated, 843 genes downregulated with age were upregulated after BMMSC treatment, and the top 20 differentially expressed genes (DEGs) in the protein-protein interaction (PPI) network were significantly enriched in oocyte meiosis and progesterone-mediated oocyte maturation pathways.
Conclusion
The BMMSCs derived from juvenile macaques can reverse ovarian ageing in elderly macaques.
Funder
the Major Science and Technology Project of Yunnan Science and Technology Plan Project
Transformation of subtotipotent stem cells based on the tree shrew model of multiple organ dysfunction syndrome
Publisher
Springer Science and Business Media LLC
Subject
Cell Biology,Biochemistry, Genetics and Molecular Biology (miscellaneous),Molecular Medicine,Medicine (miscellaneous)
Cited by
12 articles.
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