Thrombopoietin knock-in augments platelet generation from human embryonic stem cells

Author:

Zhang Leisheng,Liu Cuicui,Wang Hongtao,Wu Dan,Su Pei,Wang Mengge,Guo Jiaojiao,Zhao Shixuan,Dong Shuxu,Zhou Wen,Arakaki Cameron,Zhang Xiaobing,Zhou Jiaxi

Funder

National Basic Research Program of China

National Key Research and Development Program of China Stem Cell and Translational Research

CAMS Initiative for Innovative Medicine

Chinese National Natural Science Foundation

Natural Science Foundation of Tianjin City

PUMC Youth Fund and Fundamental Research Funds for the Central Universities

PUMC Graduate Innovation Fund

Fundamental Research Funds for the Central Research Institutes

Publisher

Springer Science and Business Media LLC

Subject

Cell Biology,Biochemistry, Genetics and Molecular Biology (miscellaneous),Molecular Medicine,Medicine (miscellaneous)

Reference33 articles.

1. Lu SJ, Li F, Yin H, Feng Q, Kimbrel EA, Hahm E, Thon JN, Wang W, Italiano JE, Cho J, Lanza R. Platelets generated from human embryonic stem cells are functional in vitro and in the microcirculation of living mice. Cell Res. 2011;21:530–45.

2. Feng Q, Shabrani N, Thon JN, Huo H, Thiel A, Machlus KR, Kim K, Brooks J, Li F, Luo C, Kimbrel EA, Wang J, Kim KS, Italiano J, J Cho SJL, Lanza R. Scalable generation of universal platelets from human induced pluripotent stem cells. Stem Cell Rep. 2014;3:817–31.

3. Moreau T, AL Evans LV, Tijssen MR, Yan Y, MW Trotter D, Howard M, Colzani M, Arumugam WHW, Dalby A, Lampela R, Bouet G, Hobbs CM, Pask DC, Payne H, Ponomaryov T, Brill A, Soranzo N, Ouwehand WH, Pedersen RA, Ghevaert C. Large-scale production of megakaryocytes from human pluripotent stem cells by chemically defined forward programming. Nat Commun. 2016;7:11208.

4. Takayama N, Nishikii H, Usui J, Tsukui H, Sawaguchi A, Hiroyama T, Eto K, Nakauchi H. Generation of functional platelets from human embryonic stem cells in vitro via ES-sacs, VEGF-promoted structures that concentrate hematopoietic progenitors. Blood. 2008;111:5298–306.

5. Nakamura S, Takayama N, Hirata S, Seo H, Endo H, Ochi K, Fujita K, Koike T, Harimoto K, Dohda T, Watanabe A, Okita K, Takahashi N, Sawaguchi A, Yamanaka S, Nakauchi H, Nishimura S, Eto K. Expandable megakaryocyte cell lines enable clinically applicable generation of platelets from human induced pluripotent stem cells. Cell Stem Cell. 2014;14:535–48.

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