APOL1 genotyping via buccal mucosa cell samples to establish risk of kidney disease-Reference-Cited by-同舟云学术

APOL1 genotyping via buccal mucosa cell samples to establish risk of kidney disease

Published:2022-10-10 Issue:1 Volume:23 Page:
ISSN:1471-2369
Container-title:BMC Nephrology
language:en
Short-container-title:BMC Nephrol

Author:

Alladagbin Dona Jeanne,da Silva Carlos Gustavo Regis,Silva Luciano Kalabric,dos-Santos Washington LC,de Sá Oliveira Geraldo Gileno

Abstract

AbstractTwo alleles (G1 and G2) of the apolipoprotein 1 gene (APOL1) predispose people of African descent to developing or accelerating the course of certain types of kidney disease. Population studies to determine the frequency of the G1 and G2 alleles are important to inform resource allocation by public health authorities. Traditionally, APOL1 genotyping is carried out in blood samples. However, sample collection, transport, and storage is cumbersome. Here we compared APOL1 genotyping in blood and buccal mucosa cell samples obtained from 23 individuals. Alleles G0 (wild), G1, and G2, as well as genotypes G0/G0, G0/G1, G1/G1, G0/G2, G1/G2, and G2/G2 were detected using both blood and buccal mucosa cells with 100% coincidence. Our data indicate that buccal mucosa cell samples may represent a suitable alternative to blood samples for APOL1 genotyping in the field.

Funder

Bahia State Research Funding Agency

Publisher

Springer Science and Business Media LLC

Subject

Nephrology

Link

https://link.springer.com/content/pdf/10.1186/s12882-022-02954-w.pdf

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