Author:
Kuang Wendong,Yan Chenghua,Zhan Zhigao,Guan Limei,Wang Jinchang,Chen Junhui,Li Jianghuai,Ma Guangqiang,Zhou Xi,Jin Liang
Abstract
Abstract
Background
Daphnis nerii cypovirus-23 (DnCPV-23) is a new type of cypovirus and has a lethal effect on the oleander hawk moth, Daphnis nerii which feeds on leave of Oleander and Catharanthus et al. After DnCPV-23 infection, the change of Daphnis nerii responses has not been reported.
Methods
To better understand the pathogenic mechanism of DnCPV-23 infection, 3rd-instar Daphnis nerii larvae were orally infected with DnCPV-23 occlusion bodies and the transcriptional responses of the Daphnis nerii midgut were analyzed 72 h post-infection using RNA-seq.
Results
The results showed that 1979 differentially expressed Daphnis nerii transcripts in the infected midgut had been identified. KEGG analysis showed that protein digestion and absorption, Toll and Imd signaling pathway were down-regulated. Based on the result, we speculated that food digestion and absorption in insect midgut might be impaired after virus infection. In addition, the down-regulation of the immune response may make D. nerii more susceptible to bacterial infections. Glycerophospholipid metabolism and xenobiotics metabolism were up-regulated. These two types of pathways may affect the viral replication and xenobiotic detoxification of insect, respectively.
Conclusion
These results may facilitate a better understanding of the changes in Daphnis nerii metabolism during cypovirus infection and serve as a basis for future research on the molecular mechanism of DnCPV-23 invasion.
Funder
the Doctoral Research Startup Project
the Doctoral Research Startup Fund
Natural Science Foundation of Jiangxi Province
Key Research and Development Program of Jiangxi Province
Key Research and Development Project of Jiangxi Province
Publisher
Springer Science and Business Media LLC
Subject
Infectious Diseases,Virology
Cited by
2 articles.
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