Author:
Li Yi,Xiao Yiwei,Li Lili,Song Yarong,Zhai Xiangjun,Liu Jianxun,Duan Zhongping,Yan Ling,Ding Feng,Liu Jia,Zhu Liguo,Jiang Jie,Zou Huaibin,Li Lingxiang,Liang Caihong,Wang Jie,Li Jie
Abstract
Abstract
Background
Previous works have observed that younger infants with chronic hepatitis B virus (HBV) infection are more responsive to antiviral treatment. However, the underlying mechanism remains unclear. In this study, the dynamic changes of HBV quasispecies in infants with immunoprophylaxis failure were investigated to provide virological explanations for clinical management on infantile antiviral therapy.
Methods
Thirteen 7-month-old infants with immunoprophylaxis failure and their mothers were enrolled from a prospective cohort, and 8 of them were followed up to 3 years old. The sequences of HBV quasispecies were analyzed by the full-length genome clone-based sequencing, and compared among mothers and their infants at different ages.
Results
The results revealed that the complexity, mutation frequency and genetic distance of HBV quasispecies decreased significantly at full-length, partial open reading frames and regulatory regions of HBV genome at nucleotide level in 7-month-old infants comparing with their mothers, whereas increased significantly to near the maternal level when infants grew up to 3 years old. Furthermore, similar changes were also found in Core, PreS2, RT and P regions of HBV genome at amino acid level, especially for potential NAs-resistant mutants in RT region and immune-escape mutants in Core and PreS2 regions.
Conclusions
This study uncovered the evolution of HBV quasispecies in infancy after mother-to-child transmission, which may provide the virological evidence for explaning that younger children are more responsive to antiviral therapy.
Funder
National Major Scientific and Technological Special Project during the Thirteenth Five-year Plan Period
National Natural Science Foundation of China
Publisher
Springer Science and Business Media LLC
Subject
Infectious Diseases,Virology
Cited by
7 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献