Author:
Ahmed Yohannis Wondwosen,Alemu Berhan Ababaw,Bekele Sisay Addisu,Gizaw Solomon Tebeje,Zerihun Muluken Fekadie,Wabalo Endriyas Kelta,Teklemariam Maria Degef,Mihrete Tsehayneh Kelemu,Hanurry Endris Yibru,Amogne Tensae Gebru,Gebrehiwot Assaye Desalegne,Berga Tamirat Nida,Haile Ebsitu Abate,Edo Dessiet Oma,Alemu Bizuwork Derebew
Abstract
AbstractNanopore sequencing has brought the technology to the next generation in the science of sequencing. This is achieved through research advancing on: pore efficiency, creating mechanisms to control DNA translocation, enhancing signal-to-noise ratio, and expanding to long-read ranges. Heterogeneity regarding epigenetics would be broad as mutations in the epigenome are sensitive to cause new challenges in cancer research. Epigenetic enzymes which catalyze DNA methylation and histone modification are dysregulated in cancer cells and cause numerous heterogeneous clones to evolve. Detection of this heterogeneity in these clones plays an indispensable role in the treatment of various cancer types. With single-cell profiling, the nanopore sequencing technology could provide a simple sequence at long reads and is expected to be used soon at the bedside or doctor’s office. Here, we review the advancements of nanopore sequencing and its use in the detection of epigenetic heterogeneity in cancer.
Publisher
Springer Science and Business Media LLC
Subject
Genetics (clinical),Developmental Biology,Genetics,Molecular Biology
Cited by
8 articles.
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