Changes in the root-associated bacteria of sorghum are driven by the combined effects of salt and sorghum development

Abstract

Abstract Background Sorghum is an important food staple in the developing world, with the capacity to grow under severe conditions such as salinity, drought, and a limited nutrient supply. As a serious environmental stress, soil salinization can change the composition of rhizosphere soil bacterial communities and induce a series of harm to crops. And the change of rhizospheric microbes play an important role in the response of plants to salt stress. However, the effect of salt stress on the root bacteria of sorghum and interactions between bacteria and sorghum remains poorly understood. Results The purpose of this study was to assess the effect of salt stress on sorghum growth performance and rhizosphere bacterial community structure. Statistical analysis confirmed that low high concentration stress depressed sorghum growth. Further taxonomic analysis revealed that the bacterial community predominantly consisted of phyla Proteobacteria, Actinobacteria, Acidobacteria, Chloroflexi, Bacteroidetes and Firmicutes in sorghum rhizosphere soil. Low salt stress suppressed the development of bacterial diversity less than high salt stress in both bulk soil and planted sorghum soil. Different sorghum development stages in soils with different salt concentrations enriched distinctly different members of the root bacteria. No obviously different effect on bacterial diversity were tested by PERMANOVA analysis between different varieties, but interactions between salt and growth and between salt and variety were detected. The roots of sorghum exuded phenolic compounds that differed among the different varieties and had a significant relationship with rhizospheric bacterial diversity. These results demonstrated that salt and sorghum planting play important roles in restructuring the bacteria in rhizospheric soil. Salinity and sorghum variety interacted to affect bacterial diversity. Conclusions In this paper, we found that salt variability and planting are key factors in shifting bacterial diversity and community. In comparison to bulk soils, soils under planting sorghum with different salt stress levels had a characteristic bacterial environment. Salinity and sorghum variety interacted to affect bacterial diversity. Different sorghum variety with different salt tolerance levels had different responses to salt stress by regulating root exudation. Soil bacterial community responses to salinity and exotic plants could potentially impact the microenvironment to help plants overcome external stressors and promote sorghum growth. While this study observed bacterial responses to combined effects of salt and sorghum development, future studies are needed to understand the interaction among bacteria communities, salinity, and sorghum growth.