Author:
Ghanchi Najia K,Mårtensson Andreas,Ursing Johan,Jafri Sana,Bereczky Sándor,Hussain Rabia,Beg Mohammad A
Abstract
Abstract
Background
The genetic diversity of Plasmodium falciparum has been extensively studied in various parts of the world. However, limited data are available from Pakistan. This study aimed to establish molecular characterization of P. falciparum field isolates in Pakistan measured with two highly polymorphic genetic markers, i.e. the merozoite surface protein 1 (msp-1)and 2 (msp-2).
Methods
Between October 2005 and October 2007, 244 blood samples from patients with symptomatic blood-slide confirmed P. falciparum mono-infections attending the Aga Khan University Hospital, Karachi, or its collection units located in Sindh and Baluchistan provinces, Pakistan were collected. The genetic diversity of P. falciparum was analysed by length polymorphism following gel electrophoresis of DNA products from nested polymerase chain reactions (PCR) targeting block 2 of msp-1 and block 3 of msp-2, including their respective allelic families KI, MAD 20, RO33, and FC27, 3D7/IC.
Results
A total of 238/244 (98%) patients had a positive PCR outcome in at least one genetic marker; the remaining six were excluded from analysis. A majority of patients had monoclonal infections. Only 56/231 (24%) and 51/236 (22%) carried multiple P. falciparum genotypes in msp-1 and msp-2, respectively. The estimated total number of genotypes was 25 msp-1 (12 KI; 8 MAD20; 5 RO33) and 33 msp-2 (14 FC27; 19 3D7/IC).
Conclusions
This is the first report on molecular characterization of P. falciparum field isolates in Pakistan with regards to multiplicity of infection. The genetic diversity and allelic distribution found in this study is similar to previous reports from India and Southeast Asian countries with low malaria endemicity.
Publisher
Springer Science and Business Media LLC
Subject
Infectious Diseases,Parasitology
Reference27 articles.
1. Yasinzai MI, Kakarsulemankhel JK: Prevalence of human malaria infection in bordering areas of East Balochistan, adjoining with Punjab: Loralai and Musakhel. J Pak Med Assoc. 2009, 59: 132-135.
2. Durrani AB, Durrani IU, Abbas N, Jabeen M: Epidemiology of cerebral malaria and its mortality. J Pak Med Assoc. 1997, 47: 213-215.
3. Rab MA, Freeman TW, Durrani N, de Poerck D, Rowland MW: Resistance of Plasmodium falciparum malaria to chloroquine is widespread in eastern Afghanistan. Ann Trop Med Parasitol. 2001, 95: 41-46. 10.1080/00034980020035906.
4. Hozhabri S, Akhtar S, Rahbar MH, Luby SP: Prevalence of plasmodium slide positivity among the children treated for malaria, Jhangara, Sindh. J Pak Med Assoc. 2000, 50: 401-405.
5. Diggs CL, Ballou WR, Miller LH: The major merozoite surface protein as a malaria vaccine target. Parasitol Today. 1993, 9: 300-302. 10.1016/0169-4758(93)90130-8.