Author:
Fontaine Albin,Pophillat Matthieu,Bourdon Stéphanie,Villard Claude,Belghazi Maya,Fourquet Patrick,Durand Claude,Lefranc Didier,Rogier Christophe,Fusai Thierry,Almeras Lionel
Abstract
Abstract
Background
Plasmodium falciparum infections could lead to severe malaria, principally in non-immune individuals as children and travellers from countries exempted of malaria. Severe malaria is often associated with the sequestration of P. falciparum-infected erythrocytes in deep micro-vascular beds via interactions between host endothelial receptors and parasite ligands expressed on the surface of the infected erythrocyte. Although, serological responses from individuals living in endemic areas against proteins expressed at surface of the infected erythrocyte have been largely studied, seldom data are available about the specific targets of antibody response from travellers.
Methods
In order to characterize antigens recognized by traveller sera, a comparison of IgG immune response against membrane protein extracts from uninfected and P. falciparum-infected red blood cells (iRBC), using immunoblots, was performed between non exposed individuals (n = 31) and briefly exposed individuals (BEI) (n = 38) to malaria transmission.
Results
Immune profile analysis indicated that eight protein bands from iRBC were significantly detected more frequently in the BEI group. Some of these antigenic proteins were identified by an original immuno-proteomic approach.
Conclusion
Collectively, these data may be useful to characterize the singular serological immune response against a primary malaria infection in individuals briefly exposed to transmission.
Publisher
Springer Science and Business Media LLC
Subject
Infectious Diseases,Parasitology
Cited by
24 articles.
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