Author:
Izzo Vincenzo,Costa Maria A,Di Fiore Renata,Duro Giovanni,Bellavia Daniele,Cascone Eleonora,Colombo Paolo,Gioviale Maria C,Barbieri Rainer
Abstract
Abstract
An inexpensive Plexiglas apparatus which allows a simple and rapid preparation of horizontal polyacrylamide gels of different dimensions for different purposes, is described. Preparation of such gels is as easy and rapid as agarose gel preparation, and polymerized polyacrylamide gels are used to fractionate proteins or small DNA fragments using a common horizontal electrophoretic tank. This apparatus was used to electrophoretically fractionate proteins or DNA for immuno-blot analyses, particularirly in the study of the allergenic response to Parietaria judaica pollen in senescence, for Southern-blot hybridizations and in the study of DNA polymorphisms.
Publisher
Springer Science and Business Media LLC
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