Annelid Distal-less/Dlx duplications reveal varied post-duplication fates

Abstract

AbstractBackgroundDlx (Distal-less) genes have various developmental roles and are widespread throughout the animal kingdom, usually occurring as single copy genes in non-chordates and as multiple copies in most chordate genomes. While the genomic arrangement and function of these genes is well known in vertebrates and arthropods, information about Dlx genes in other organisms is scarce. We investigate the presence of Dlx genes in several annelid species and examine Dlx gene expression in the polychaetePomatoceros lamarckii.ResultsTwo Dlx genes are present inP. lamarckii, Capitella teletaandHelobdella robusta. TheC. teletaDlx genes are closely linked in an inverted tail-to-tail orientation, reminiscent of the arrangement of vertebrate Dlx pairs, and gene conversion appears to have had a role in their evolution. TheH. robustaDlx genes, however, are not on the same genomic scaffold and display divergent sequences, while, if theP. lamarckiigenes are linked in a tail-to-tail orientation they are a minimum of 41 kilobases apart and show no sign of gene conversion. No expression inP. lamarckiiappendage development has been observed, which conflicts with the supposed conserved role of these genes in animal appendage development. These Dlx duplications do not appear to be annelid-wide, as the polychaetePlatynereis dumeriliilikely possesses only one Dlx gene.ConclusionsOn the basis of the currently accepted annelid phylogeny, we hypothesise that one Dlx duplication occurred in the annelid lineage after the divergence ofP. dumeriliifrom the other lineages and these duplicates then had varied evolutionary fates in different species. We also propose that the ancestral role of Dlx genes is not related to appendage development.