The identification of allergen proteins in sugar beet (Beta vulgaris) pollen causing occupational allergy in greenhouses

Author:

Luoto Susanne,Lambert Wietske,Blomqvist Anna,Emanuelsson Cecilia

Abstract

Abstract Background During production of sugar beet (Beta vulgaris) seeds in greenhouses, workers frequently develop allergic symptoms. The aim of this study was to identify and characterize possible allergens in sugar beet pollen. Methods Sera from individuals at a local sugar beet seed producing company, having positive SPT and specific IgE to sugar beet pollen extract, were used for immunoblotting. Proteins in sugar beet pollen extracts were separated by 1- and 2-dimensional electrophoresis, and IgE-reactive proteins analyzed by liquid chromatography tandem mass spectrometry. Results A 14 kDa protein was identified as an allergen, since IgE-binding was inhibited by the well-characterized allergen Che a 2, profilin, from the related species Chenopodium album. The presence of 17 kDa and 14 kDa protein homologues to both the allergens Che a 1 and Che a 2 were detected in an extract from sugar beet pollen, and partial amino acid sequences were determined, using inclusion lists for tandem mass spectrometry based on homologous sequences. Conclusion Two occupational allergens were identified in sugar beet pollen showing sequence similarity with Chenopodium allergens. Sequence data were obtained by mass spectrometry (70 and 25%, respectively for Beta v 1 and Beta v 2), and can be used for cloning and recombinant expression of the allergens. As for treatment of Chenopodium pollinosis, immunotherapy with sugar beet pollen extracts may be feasible.

Publisher

Springer Science and Business Media LLC

Subject

Molecular Biology,Immunology,Immunology and Allergy

Cited by 10 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. A hidden allergen of anaphylaxis: Beetroot;Revue Française d'Allergologie;2023-05

2. Is exposure to pollen a risk factor for moderate and severe asthma exacerbations?;Allergy;2023-04-10

3. Food anaphylaxis due to boiled beetroot;The Journal of Allergy and Clinical Immunology: In Practice;2020-10

4. Analysis of Pollen Allergens in Lily by Transcriptome and Proteome Data;International Journal of Molecular Sciences;2019-11-24

5. Beta vulgaris;Edible Medicinal and Non-Medicinal Plants;2016

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