Author:
Zheng Wen Yin,Zhu Zhe Yi,Sami Abdul,Sun Meng Yuan,Li Yong,Hu Jian,Qian Xing Zhi,Ma Jin Xu,Wang Mei Qi,Yu Yan,Zhang Fu Gui,Zhou Ke Jin,Zhu Zong He
Abstract
AbstractBreeding rapeseed varieties with more main inflorescence siliques is an idea for developing rapeseed varieties that are suitable for light and simplified cultivation. The Brassica napus exhibited cluster bud of the main inflorescence (Bnclib) gene. At the fruiting stage, the main inflorescence had more siliques, higher density, and more main inflorescences. Moreover, the top of the main inflorescence bifurcated. Genetic analysis showed that the separation ratio between Bnclib and the wild type in the F2 generation was 3:1, which indicated that the trait was a single-gene-dominant inheritance. Among the 24 candidate genes, only one gene, BnaA03g53930D, showed differential expression between the groups (False discovery rate, FDR ≤ 0.05, |log2FC|≤ 1). qPCR verification of the BnaA03g53930D gene between Huyou 17 and its Bnclib near-isogenic line showed that BnaA03g53930D was significantly differentially expressed in the stem tissue of Huyou 17 and its Bnclib near-isogenic line (Bnclib NIL). The determination of gibberellin (GA), brassinolide (BR), cytokinin (CTK), jasmonic acid (JA), growth hormone (IAA), and strigolactone (SL) content in the shoot apex of Huyou 17 by Bnclib NIL and wild type showed that all six hormones significantly differed between the Bnclib NIL and Huyou 17. It is necessary to conduct further research on the interactions between JA and the other five hormones and the main inflorescence bud clustering in B. napus.
Publisher
Springer Science and Business Media LLC
Reference40 articles.
1. Antoniadi I, Plačková L, Simonovik B, Doležal K, Turnbull C, Ljung K, Novák O. Cell-type-specific cytokinin distribution within the Arabidopsis primary root apex. Plant Cell. 2015;27(7):1955–67.
2. Binenbaum J, Weinstain R, Shani E. Gibberellin localization and transport in plants. Trends Plant Sci. 2018;23(5):410–21.
3. Chen W, Zhang Y, Liu X, Chen B, Tu J, Tingdong F. Detection of QTL for six yield-related traits in oilseed rape (Brassica napus) using DH and immortalized F2 populations. Theor Appl Genet. 2007;115(6):849–58.
4. Deshmukh UC, Saxena RR, Xalxo MS, Sharma D, Verulkar SB. Hybrid purity testing in rice (Oryza sativa L.) using microsatellite markers. Electron J Plant Breed. 2013;4(1):1021–6.
5. Ding J, Mao LJ, Yuan BF, Feng YQ. A selective pretreatment method for determination of endogenous active brassinosteroids in plant tissues: double layered solid phase extraction combined with boronate affinity polymer monolith microextraction. Plant Methods. 2013;9(1):1–9.
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献