The Bursaphelenchus xylophilus effector BxML1 targets the cyclophilin protein (CyP) to promote parasitism and virulence in pine

Abstract

AbstractBackgroundBursaphelenchus xylophilusis the causal agent of pine wilt disease (PWD) that has caused enormous ecological and economic losses in China. The mechanism in the interaction between nematodes and pine remains unclear. Plant parasitic nematodes (PPNs) secrete effectors into host plant tissues. However, it is poorly studied that role of effector in the infection of pine wood nematode (PWN).ResultsWe cloned, characterized and functionally validated theB. xylophiluseffector BxML1, containing an MD-2-related lipid-recognition (ML) domain. This protein inhibits immune responses triggered by the molecular pattern BxCDP1 ofB. xylophilus. An insitu hybridization assay demonstrated that BxML1 was expressed mainly in the dorsal glands and intestine ofB. xylophilus. Subcellular localization analysis showed the presence of BxML1 in the cytoplasm and nucleus. Furthermore, number ofB. xylophilusand morbidity of pine were significantly reduced inPinus thunbergiiinfected withB. xylophiluswhen BxML was silenced. Using yeast two-hybrid (Y2H) and coimmunoprecipitation (CoIP) assays, we found that the BxML1 interacts with cyclophilin protein PtCyP1 inP. thunbergii.ConclusionsThis study illustrated that BxML1 plays a critical role in theB. xylophilus–plant interaction and virulence ofB. xylophilus.