Characterization of the wheat VQ protein family and expression of candidate genes associated with seed dormancy and germination
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Published:2022-03-15
Issue:1
Volume:22
Page:
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ISSN:1471-2229
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Container-title:BMC Plant Biology
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language:en
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Short-container-title:BMC Plant Biol
Author:
Cheng Xinran,Gao Chang,Liu Xue,Xu Dongmei,Pan Xu,Gao Wei,Yan Shengnan,Yao Hui,Cao Jiajia,Min Xiaoyu,Lu Jie,Chang Cheng,Zhang Haiping,Ma Chuanxi
Abstract
Abstract
Background
Seed dormancy and germination determine wheat resistance to pre-harvest sprouting and thereby affect grain yield and quality. Arabidopsis VQ genes have been shown to influence seed germination; however, the functions of wheat VQ genes have not been characterized.
Results
We identified 65 TaVQ genes in common wheat and named them TaVQ1–65. We identified 48 paralogous pairs, 37 of which had Ka/Ks values greater than 1, suggesting that most TaVQ genes have experienced positive selection. Chromosome locations, gene structures, promoter element analysis, and gene ontology annotations of the TaVQs showed that their structures determined their functions and that structural changes reflected functional diversity. Transcriptome-based expression analysis of 62 TaVQ genes and microarray analysis of 11 TaVQ genes indicated that they played important roles in diverse biological processes. We compared TaVQ gene expression and seed germination index values among wheat varieties with contrasting seed dormancy and germination phenotypes and identified 21 TaVQ genes that may be involved in seed dormancy and germination.
Conclusions
Sixty-five TaVQ proteins were identified for the first time in common wheat, and bioinformatics analyses were used to investigate their phylogenetic relationships and evolutionary divergence. qRT-PCR data showed that 21 TaVQ candidate genes were potentially involved in seed dormancy and germination. These findings provide useful information for further cloning and functional analysis of TaVQ genes and introduce useful candidate genes for the improvement of PHS resistance in wheat.
Funder
National Natural Science Foundation of China
Publisher
Springer Science and Business Media LLC
Reference70 articles.
1. Brenchley R, Spannagl M, Pfeifer M, Barker GLA, DAmore R, Allen AM, McKenzie N, Kramer M, Kerhornou A, Dan B. Analysis of the bread wheat genome using whole genome shotgun sequencing. Nature. 2012;491:705–10. 2. Luo MC, Gu YQ, Puiu D, Wang H, Twardziok SO, Deal KR, Huo N, Zhu T, Wang L, Wang Y, McGuire PE, Liu S, Long H, Ramasamy RK. Genome sequence of the progenitor of the wheat D genome Aegilops tauschii. Nature. 2007;551:498–502. 3. Ling H, Ma B, Shi X, Liu H, Dong L, Sun H, Cao Y, Gao Q, Zheng S, Li Y, Yu Y, Du H, Qi M, Li Y, Lu H, Yu H, Cui Y, Wang N. Genome sequence of the progenitor of wheat A subgenome Triticum urartu. Nature. 2018;557:424–8. 4. Pecher P, Eschen-Lippold L, Herklotz S, Kuhle K, Naumann K, Bethke G, Uhrig J, Weyhe M, Scheel D, Lee J. The Arabidopsis thaliana mitogen-activated protein kinases MPK3 and MPK6 target a subclass of ‘VQ-motif’-containing proteins to regulate immune responses. New Phy. 2014;203:592–606. 5. Kim DY, Kwon SI, Choi C, Lee H, Ahn I, Park SR, Bae SC, Lee SC, Hwang DJ. Expression analysis of rice VQ genes in response to biotic and abiotic stresses. Gene. 2013;529:208–14.
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