Author:
Tayebi Zahra,Moghaddam Mahtab,Mahmoodi Mohammad,Kazempour-Osaloo Shahrokh
Abstract
AbstractThe Irano-Turanian region is one of the largest floristic regions in the world and harbors a high percentage of endemics, including cushion-like and dwarf-shrubby taxa. Onobrychis cornuta is an important cushion-forming element of the subalpine/alpine flora of the Irano-Turanian floristic region. To specify the genetic diversity among the populations of this species (including individuals of O. elymaitica), we employed nrDNA ITS and two noncoding regions of plastid DNA (rpl32-trnL(UAG) and trnT(UGU)-trnL(UAA)). The most striking feature of O. cornuta assemblages was the unexpectedly high nucleotide diversity in both the nDNA and cpDNA dataset. In the analyses of nuclear and plastid regions, 25 ribotypes and 42 haplotypes were found among 77 and 59 accessions, respectively, from Iran, Turkey, and Afghanistan. Network analysis of the datasets demonstrated geographic differentiation within the species. Phylogenetic analyses of all dataset retrieved O. cornuta as a non-monophyletic species due to the inclusion of O. elymaitica, comprising four distinct lineages. In addition, our analyses showed cytonuclear discordance between both nuclear and plastid topologies regarding the position of some O. cornuta individuals. The underlying causes of this inconsistency remain unclear. However, we speculate that chloroplast capture, incomplete lineage sorting, and introgression were the main reasons for this event. Furthermore, molecular dating analysis indicated that O. cornuta originated in the early Pliocene (around 4.8 Mya) and started to diversify throughout the Pliocene and in particular the Pleistocene. Moreover, O. elymaitica was reduced to a subspecific rank within the species.
Publisher
Springer Science and Business Media LLC
Reference87 articles.
1. Aubert S, Boucher F, Lavergne S, Renaud J, Choler P. 1914–2014: a revised worldwide catalogue of cushion plants 100 years after Hauri and Schröter. Alp Bot. 2014;124:59–70. https://doi.org/10.1007/s00035-014-0127-x
2. Boucher FC, Lavergne S, Basile M, Choler P, Aubert S. Evolution and biogeography of the cushion life form in angiosperms. Perspec. Plant Ecol Evol Syst. 2016;20:22–31. https://doi.org/10.1016/j.ppees.2016.03.002
3. Körner C. Alpine plant life: functional plant ecology of high mountain ecosystems. 3rd ed. Springer: Nature Switzerland AG, Cham; 2021.
4. Reid AM, Lamarque LJ, Lortie CJ. A systematic review of the recent ecological literature on cushion plants: champions of plant facilitation. Web Ecol. 2010;10:44–9. https://doi.org/10.5194/we-10-44-2010
5. POWO. Plants of the World Online. Facilitated by the Royal Botanic Gardens, Kew. Published on the Internet. 2023. http://www.plantsoftheworldonline.org/ Accessed 26 September 2023.