Author:
Liu Hongbo,Zhu Jinbo,Zhang Bingxin,Li Qingyang,Liu Cui,Huang Qian,Cui Peng
Abstract
Abstract
Background
The early allopolyploid Brassica napus was a hybrid of two Brassica species, that had undergone a whole genome duplication event followed by genome restructuring, including deletions and small scale duplications. A large number of homologous genes appeared functional divergence during species domestication. Due to the high conservation of de novo glycerolipid biosynthesis, multiple homologues of glycerol-3-phosphate acyltransferases (GPATs) have been found in B. napus. Moreover, the functional variances among these homologous GPAT-encoding genes are unclear.
Results
In this study, four B. napus homologous genes encoding glycerol-3-phosphate acyltransferase 9 (BnaGPAT9) were characterized. Although a bioinformatics analysis indicated high protein sequence similarity, the homologues demonstrated tissue-specific expression patterns and functional divergence. Yeast genetic complementation assays revealed that BnaGPAT9-A1/C1 homologues but not BnaGPAT9-A10/C9 homologues encoded functional GPAT enzymes. Furthermore, a single nucleotide polymorphism of BnaGPAT9-C1 that occurred during the domestication process was associated with enzyme activity and contributed to the fatty acid composition. The seed-specific expression of BnGPAT9-C11124A increased the erucic acid content in the transformant seeds.
Conclusions
This study revealed that BnaGPAT9 gene homologues evolved into functionally divergent forms with important roles in erucic acid biosynthesis.
Funder
National Natural Science Foundation of China
Natural Science Foundation of Zhejiang Province
Scientific Research Foundation of Zhejiang A and F University
Collaborative Innovation Center for Modern Crop Production cosponsored by the Province and Ministry
Publisher
Springer Science and Business Media LLC
Reference28 articles.
1. United States Department of Agriculture. Oilseeds: World Markets and Trade. https://apps.fas.usda.gov/psdonline/circulars/oilseeds.pdf. 2023; [Accessed March 8, 2023].
2. Ohlrogge J, Browse J. Lipid biosynthesis. Plant Cell. 1995;7:957–70.
3. Weselake RJ, Shah S, Tang M, Quant PA, Snyder CL, Furukawa-Stoffer TL, Zhu W, Taylor DC, Zou J, Kumar A, et al. Metabolic control analysis is helpful for informed genetic manipulation of oilseed rape (Brassica napus) to increase seed oil content. J Exp Bot. 2008;59(13):3543–9. https://doi.org/10.1093/jxb/ern206.
4. Zheng Z, Xia Q, Dauk M, Shen W, Selvaraj G, Zou J. Arabidopsis AtGPAT1, a member of the membrane-bound glycerol-3-phosphate acyltransferase gene family, is essential for tapetum differentiation and male fertility. Plant Cell. 2003;15(8):1872–87. https://doi.org/10.1105/tpc.012427.
5. Wendel AA, Lewin TM, Coleman RA. Glycerol-3-phosphate acyltransferases: rate limiting enzymes of triacylglycerol biosynthesis. Biochim Biophys Acta. 2009;1791(6):501–6. https://doi.org/10.1016/j.bbalip.2008.10.010.
Cited by
3 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献