Author:
He Lei,Wang Hairong,Yu Zuhua,Liao Chengshui,Ding Ke,Zhang Cai,Yu Chuan,Zhang Chunjie
Abstract
Abstract
Background
Newcastle disease virus (NDV) strain ZM10, a typical enterotropic avirulent vaccine strain, has been widely used in China for chickens against Newcastle disease. To elucidate its enterotropic mechanism and develop recombiant multivalent vaccines based on it, the reverse genetics system for NDV ZM10 is an indispensable platform.
Results
A full-length cDNA clone of NDV ZM10 and three supporting plasmids were constructed using the ligation-independent cloning method. Recombinant NDV rZM10 was successfully rescued after these plasmids were co-transfected into BHK-21 cells. Besides, the recombinant virus rZM10-RFP encoding the red fluorescent protein was generated by inserting the RFP gene into the full-length clone of NDV between the P and M genes. These rescued viruses were genetically and biologically identical to the parental strain and showed similar growth kinetics.
Conclusion
The recovery system of NDV ZM10 strain was established, and can be used as a foundation for research on the enterotropic mechanism and development of multivalent vaccines against viral diseases of livestock and poultry.
Funder
the science and technology research project of Henan province
the key project of science and technology research of Education Department of Henan Province
The Science and Technology Research Program of Henan Province
Publisher
Springer Science and Business Media LLC
Cited by
2 articles.
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