Author:
Kim Dong-Ern,Lee Ji-Hye,Ji Kuk-Bin,Park Kang-Sun,Kil Tae-Young,Koo Okjae,Kim Min-Kyu
Abstract
Abstract
Background
Canine cloning technology based on somatic cell nuclear transfer (SCNT) combined with genome-editing tools such as CRISPR-Cas9 can be used to correct pathogenic mutations in purebred dogs or to generate animal models of disease.
Results
We constructed a CRISPR-Cas9 vector targeting canine DJ-1. Genome-edited canine fibroblasts were established using vector transfection and antibiotic selection. We performed canine SCNT using genome-edited fibroblasts and successfully generated two genome-edited dogs. Both genome-edited dogs had insertion-deletion mutations at the target locus, and DJ-1 expression was either downregulated or completely repressed.
Conclusion
SCNT successfully produced genome-edited dogs by using the CRISPR-Cas9 system for the first time.
Publisher
Springer Science and Business Media LLC
Cited by
6 articles.
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