Engineering Bacillus pumilus alkaline serine protease to increase its low-temperature proteolytic activity by directed evolution
Author:
Funder
the National Science of foundation of China
Talent Start-up Fund by the Hubei University of Medicine
Publisher
Springer Science and Business Media LLC
Subject
Biotechnology
Link
http://link.springer.com/content/pdf/10.1186/s12896-018-0451-0.pdf
Reference44 articles.
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3. Bryan PN. Protein engineering of subtilisin. Biochim Biophys Acta. 2000;1543:203–22.
4. Polgár L. The catalytic triad of serine peptidases. Cell Mol Life Sci. 2005;62:2161–72.
5. Feller G. Psychrophilic enzymes: from folding to function and biotechnology. Scientica. 2013:Article ID 512840.
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