Abstract
Abstract
Background
Promoters that confer expression in fruit tissues are important tools for genetic engineering of fruit quality traits, yet few fruit-specific promoters have been identified, particularly for citrus fruit development.
Results
In this study, we report five citrus fruit-specific/preferential promoters for genetic engineering. Additionally, we have characterized a novel fruit-preferential promoter from plum. Genes specifically expressed in fruit tissues were selected and their isolated promoter regions were fused with the GUSPlus reporter gene for evaluation in transgenic plants. Stable transformation in Micro-Tom tomato demonstrated that the candidate promoter regions exhibit differing levels of expression and with varying degrees of fruit specificity.
Conclusions
Among the five candidate citrus promoters characterized in this study, the CitSEP promoter showed a fruit-specific expression pattern, while the CitWAX and CitJuSac promoters exhibited high fruit-preferential expression with strong activity in the fruit, weak activity in floral tissues and low or undetectable activity in other tissues. The CitVO1, CitUNK and PamMybA promoters, while exhibiting strong fruit-preferential expression, also showed consistent weak but detectable activity in leaves and other vegetative tissues. Use of these fruit specific/preferential promoters for genetic engineering can help with precise expression of beneficial genes and help with accurate prediction of the activity of new genes in host fruit plants.
Funder
California Citrus Research Board
USDA-ARS
Publisher
Springer Science and Business Media LLC
Reference69 articles.
1. Rai MK, Shekhawat NS. Recent advances in genetic engineering for improvement of fruit crops. Plant Cell Tiss Org. 2014;116:1..
2. Klee HJ. Improving the flavor of fresh fruits: genomics, biochemistry, and biotechnology. New Phytol. 2010;187(1):44–56.
3. Parmar N, Singh KH, Sharma D, Singh L, Kumar P, Nanjunda J, Khan YJ, Chauhan DK, Thakur AK. Genetic engineering strategies for biotic and abiotic stress tolerance and quality enhancement in horticultural crops: a comprehensive review. Biotech. 2017;7(4):239.
4. Gomez-Lim MA, Litz RE. Genetic transformation of perennial tropical fruits. In Vitro Cell Dev Biol Plant. 2004;40:442–9.
5. Petri C, Burgos L. Transformation of fruit trees. Useful breeding tool or continued future prospect? Transgenic Res. 2005;14:15–26.
Cited by
4 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献