A restriction-free method for gene reconstitution using two single-primer PCRs in parallel to generate compatible cohesive ends
Author:
Funder
agricultural university of hebei
Agricultural University of Hebei
Publisher
Springer Science and Business Media LLC
Subject
Biotechnology
Link
http://link.springer.com/content/pdf/10.1186/s12896-017-0346-5.pdf
Reference24 articles.
1. Luft JR, Snell EH, Detitta GT. Lessons from high-throughput protein crystallization screening: 10 years of practical experience. Expert Opin Dru Discov. 2011;6(5):465–80.
2. Green MR, Sambrook J. Molecular cloning: a laboratory manual, fourth edition edn. NY Cold Spring Harbor Laboratory Press: Cold Spring Harbor; 2012.
3. van den Ent F, Lowe J. RF cloning: a restriction-free method for inserting target genes into plasmids. J Biochem Biophys Methods. 2006;67(1):67–74.
4. Felfoldi F, Kupihar Z, Bottka S, Puskas LG. Direct ligation of human CD4 polymerase chain reaction fragment into vectors at specific restriction sites with positional heterostagger cloning. Anal Biochem. 1997;253(2):275–7.
5. Zhou MY, Gomez-Sanchez CE. Universal TA cloning. Curr Issues Mol Biol. 2000;2(1):1–7.
Cited by 16 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献
1. A selection of Golden Gate vectors to simplify recombinant protein production inEscherichia coli;2024-02-13
2. Production of CFTR Mutant Gene Model by Homologous Recombination System;CELL J;2022
3. AdenoBuilder: A platform for the modular assembly of recombinant adenoviruses;STAR Protocols;2022-03
4. Methods for enzyme library creation: Which one will you choose?;BioEssays;2021-07-15
5. Crystallization and preliminary X-ray diffraction studies of melibiose permease;2020-06-29
1.学者识别学者识别
2.学术分析学术分析
3.人才评估人才评估
"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370
www.globalauthorid.com
TOP
Copyright © 2019-2024 北京同舟云网络信息技术有限公司 京公网安备11010802033243号 京ICP备18003416号-3