Impact of influenza virus infection on lung microbiome in adults with severe pneumonia
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Published:2023-06-02
Issue:1
Volume:22
Page:
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ISSN:1476-0711
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Container-title:Annals of Clinical Microbiology and Antimicrobials
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language:en
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Short-container-title:Ann Clin Microbiol Antimicrob
Author:
Zhou Yiguo,Du Juan,Wu Jing-Qin,Zhu Quan-Rong,Xie Ming-Zhu,Chen Lin-Yi,Liu Ya-Qiong,Li Wei,Zhou Ting-Fa,Lu Qing-Bin
Abstract
Abstract
Background
Bacterial and viral infections are commonly implicated in the development of pneumonia. We aimed to compare the diversity and composition of lung bacteria among severe pneumonia patients who were influenza virus positive (IFVP) and influenza virus negative (IFVN).
Methods
Bronchoalveolar lavage fluid specimens were procured from patients diagnosed with severe pneumonia to investigate the microbiome utilizing 16S-rDNA sequencing. The alpha diversity of the microbiome was evaluated employing Chao1, Shannon, and Simpson indexes, while the beta diversity was assessed using principal component analysis and principal coordinate analysis. Linear discriminant analysis effect size (LEfSe) was employed to determine the taxonomic differences between the IFVP and IFVN groups.
Results
A total of 84 patients with 42 in the IFVP group and 42 in the IFVN group were enrolled. Slightly higher indexes of Shannon and Simpson were observed in the IFVP group without statistically significant difference. The dominant bacterial genera were Streptococcus, Klebsiella, Escherichia-Shigella in the IFVN group and Acinetobacter, Streptococcus, Staphylococcus in the IFVP group. Streptococcus pneumoniae and Acinetobacter baumannii were the most abundant species in the IFVN and IFVP groups, respectively. LEfSe analysis indicated a greater abundance of Klebsiella in the IFVN group.
Conclusions
Individuals with severe pneumonia infected with IFV exhibit heightened susceptibility to certain bacteria, especially Acinetobacter baumannii, and the underlying mechanism of the interaction between IFV and Acinetobacter baumannii in the progression of pneumonia needs further investigation.
Funder
China Mega-Project for Infectious Diseases grants
Fundamental Research Funds for the Central Universities and Peking University Health Science Center
Peking University Medicine Fund of Fostering Young Scholars’ Scientific & Technological Innovation
Joint Research Fund for Beijing Natural Science Foundation and Haidian Original Innovation
Publisher
Springer Science and Business Media LLC
Subject
Infectious Diseases,Microbiology (medical),General Medicine
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