Clinical and molecular characteristics of methicillin-resistant Staphylococcus aureus in bone and joint infection among children

Abstract

Abstract Objective To investigate the characteristics of Methicillin-Resistant Staphylococcus aureus (MRSA) in bone and joint infection (BJI) among children. Methods A total of 338 patients diagnosed with BJI from 2013 to 2022 in Children’s Hospital of Fudan University were enrolled. Demographic information, microbiology culture results and laboratory findings, including white blood counts (WBC), C-reactive protein (CRP), procalcitonin (PCT), interleukin-6 (IL-6), and erythrocyte sedimentation rate (ESR) were collected and analyzed. MRSA was confirmed by antimicrobial susceptibility testing. Other MRSA-caused infections were randomly selected for comparison. Twenty-three virulence and antimicrobial resistance (AMR) genes were screened for MRSA strains. Multilocus sequence typing (MLST) and Staphylococcal protein A (spa) typing were performed using PCR amplification and sequencing. Results Of the identified pathogens in BJI, MRSA accounted for 21.0% (47/224). Patients with BJI had high levels of initial CRP, white blood cell count (WBC) and IL-6. ST59 (43.9%) and t437 (37.6%) were the main MRSA subtypes isolated from the children. The major genotypes in BJI were ST59-t437 (29.8%) and ST22-t309 (14.9%), with high carriage of hemolysins including hla (94.4–100%), hlb (66.2–93.3%), and hld (100%). Notably, Panton–Valentine leukocidin (pvl) had a high prevalence (53.3%) in ST22-t309-MRSA. Other virulence genes including tst, seg and sei were more commonly detected in ST22-t309-MRSA (40.0–46.7%) than in ST59-t437-MRSA (4.2–9.9%). High-carriage AMR genes in MRSA included aph(3ʹ)/III (66.7–80%), ermB (57.5–73.3%) and ermC (66.7–78.9%). MRSA presented high-resistance to erythromycin (52.0–100%) and clindamycin (48.0–92.5%), different genotypes displayed variation in their susceptibilities to antibiotics. Conclusions The major MRSA genotype in BJI was ST59-t437, followed by ST22-t309, with a higher prevalence of the pvl gene. Continuous surveillance of pvl-positive ST22-t309-MRSA in pediatric BJI infections is thus required.