Author:
Cheng Weixia,Chen Jinan,Xu Ziqian,Yu Jiemei,Huang Canping,Jin Miao,Li Huiying,Zhang Ming,Jin Yu,Duan Zhao-jun
Abstract
Abstract
Background
Human bocavirus 2(HBoV2) and other human bocavirus species (HBoV, HBoV3, and HBoV4) have been discovered recently. But the precise phylogenetic relationships among these viruses are not clear yet.
Methods
We collected 632 diarrhea and 162 healthy children in Lanzhou, China. Using PCR, Human bocavirus (HBoV), HBoV2, HBoV3 and HBoV4 were screened. The partial genes of NS, NP1 and VP, and two nearly complete sequences of HBoV2 were obtained.
Result
Phylogenetic analysis showed the different genes of HBoV2 strain were homogenous with different reference strains. HBoV3 may be a recombinant derived from HBoV and HBoV4. We also observed that the VP1 and VP2 region of HBoV3 is as similar to HBoV2 as to HBoV4.
Conclusions
A single genetic lineage of HBoV2 is circulating in children with and without gastroenteritis in Lanzhou, China. Current evidence in this study was not enough to support recombination between HBoV2 strains, and HBoV3 may be a recombinant between HBoV and the common ancestor of HBoV2 and HBoV4.
Publisher
Springer Science and Business Media LLC
Reference15 articles.
1. Allander T, Tammi MT, Eriksson M, Bjerkner A, Tiveljung-Lindell A, Andersson B: Cloning of a human parvovirus by molecular screening of respiratory tract samples. Proc Natl Acad Sci USA. 2005, 102: 12891-6. 10.1073/pnas.0504666102.
2. Kapoor A, Slikas E, Simmonds P, Chieochansin T, Naeem A, Shaukat S, Alam MM, Sharif S, Angez M, Zaidi S, Delwart E: A newly identified bocavirus species in human stool. J Infect Dis. 2009, 199: 196-200. 10.1086/595831.
3. Arthur JL, Higgins GD, Davidson GP, Givney RC, Ratcliff RM: A novel bocavirus associated with acute gastroenteritis in Australian children. PLoS Pathog. 2009, 5: e1000391-10.1371/journal.ppat.1000391.
4. Kapoor A, Simmonds P, Slikas E, Li L, Bodhidatta L, Sethabutr O, Triki H, Bahri O, Oderinde BS, Baba MM, Bukbuk DN, Besser J, Bartkus J, Delwart E: Human bocaviruses are highly diverse, dispersed, recombination prone, and prevalent in enteric infections. J Infect Dis. 2010, 201: 1633-43. 10.1086/652416.
5. Suzuki M, Yoto Y, Ishikawa A, Tsutsuimi H: Analysis of nucleotide sequences of human parvovirus B19 genome reveals two different modes of evolution, a gradual alteration and a sudden replacement: a retrospective study in Sapporo, Japan from 1980 to 2008. J Virol. 2009
Cited by
33 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献