Trajectories in nitrogen availability during forest secondary succession: illustrated by foliar δ15N

Abstract

Abstract Background Forest succession is an important ecological process and has been studied for more than a century. However, changes in nitrogen (N) availability during succession remain unclear as they may lead to either N saturation or N limitation. Here, we propose a conceptual model to illustrate changes in N availability during four stages of secondary succession using the natural abundance of 15N in plant leaves (foliar δ15N). We predicted that N availability would decline in the early stages of succession and then increase in late stages, coinciding with the changes in foliar δ15N, with the inflection point varying in different climate zones. Data on foliar δ15N from 16 succession sequences were synthesized to explore changes in N availability during forest succession. Results The compiled data were consistent with the proposed conceptual model. Foliar δ15N in boreal and temperate forests decreased significantly in the first two stages of succession (estimated to last at least 66 years in temperate forests), at a rate of 0.18‰ and 0.38‰ per decade, respectively, and decreased slightly in tropical forests in the first 23 years. Foliar δ15N is projected to increase in later stages in all forests, which is supported by observations in both temperate and tropical forests. The inflection points of N availability when N limitation peaked during succession were different in different climate zones, implying different ecosystem N turnovers. Conclusions Our study reconciles the controversies regarding changes in N availability during forest secondary succession. Our findings are also useful for predicting the recovery of N and carbon accumulation during succession. Nonetheless, studies on forest secondary succession using foliar δ15N have thus far been limited, and more research should be conducted to further verify the conceptual model proposed here.