Abstract
Abstract
Background
Succinic acid (SA), a valuable chemical compound with a broad range of industrial uses, has become a subject of global interest in recent years. The bio-based production of SA by highly efficient microbial producers from renewable feedstock is significantly important, regarding the current trend of sustainable development.
Results
In this study, a novel bacterial strain, LU2, was isolated from cow rumen and recognized as an efficient producer of SA from lactose. Proteomic and genetic identifications as well as phylogenetic analysis were performed, and strain LU2 was classified as an Enterobacter aerogenes species. The optimal conditions for SA production were 100 g/L lactose, 10 g/L yeast extract, and 20% inoculum at pH 7.0 and 34 °C. Under these conditions, approximately 51.35 g/L SA with a yield of 53% was produced when batch fermentation was conducted in a 3-L stirred bioreactor. When lactose was replaced with whey permeate, the highest SA concentration of 57.7 g/L was achieved with a yield and total productivity of 62% and 0.34 g/(L*h), respectively. The highest productivity of 0.67 g/(L*h) was observed from 48 to 72 h of batch fermentation, when E. aerogenes LU2 produced 16.23 g/L SA.
Conclusions
This study shows that the newly isolated strain E. aerogenes LU2 has great potential as a new biocatalyst for producing SA from whey permeate.
Publisher
Springer Science and Business Media LLC
Subject
Management, Monitoring, Policy and Law,General Energy,Renewable Energy, Sustainability and the Environment,Applied Microbiology and Biotechnology,Biotechnology
Reference48 articles.
1. Zeikus JG, Jain MK, Elankovan P. Biotechnology of succinic acid production and markets for derived industrial products. Appl Microbiol Biotechnol. 1999;51(5):545–52.
2. Werpy T, Petersen G. Top value added chemicals from biomass volume I—results of screening for potential candidates from sugars and synthesis gas energy efficiency and renewable energy. Washington D.C: National Renewable Energy Lab; 2004.
3. Beauprez JJ, De Mey M, Soetaert WK. Microbial succinic acid production: natural versus metabolic engineered producers. Process Biochem. 2010;45(7):1103–14.
4. McKinlay JB, Vieille C, Zeikus JG. Prospects for a bio-based succinate industry. Appl Microbiol Biotechnol. 2007;76:727–40.
5. Wan C, Li Y, Shahbazi A, Xiu S. Succinic acid production from cheese whey using Actinobacillus succinogenes 130 Z. Appl Biochem Biotechnol. 2008;145(1–3):111–9.
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