Author:
Wang Qiang,Zhang Xian,Ren Kexin,Han Rumeng,Lu Ruiqi,Bao Teng,Pan Xuewei,Yang Taowei,Xu Meijuan,Rao Zhiming
Abstract
Abstract
Background
Acetoin (AC) is a vital platform chemical widely used in food, pharmaceutical and chemical industries. With increasing concern over non-renewable resources and environmental issues, using low-cost biomass for acetoin production by microbial fermentation is undoubtedly a promising strategy.
Results
This work reduces the disadvantages of Bacillus subtilis during fermentation by regulating genes involved in spore formation and autolysis. Then, optimizing intracellular redox homeostasis through Rex protein mitigated the detrimental effects of NADH produced by the glycolytic metabolic pathway on the process of AC production. Subsequently, multiple pathways that compete with AC production are blocked to optimize carbon flux allocation. Finally, the population cell density-induced promoter was used to enhance the AC synthesis pathway. Fermentation was carried out in a 5-L bioreactor using bagasse lignocellulosic hydrolysate, resulting in a final titer of 64.3 g/L, which was 89.5% of the theoretical yield.
Conclusions
The recombinant strain BSMAY-4-PsrfA provides an economical and efficient strategy for large-scale industrial production of acetoin.
Publisher
Springer Science and Business Media LLC
Subject
Management, Monitoring, Policy and Law,Energy (miscellaneous),Applied Microbiology and Biotechnology,Renewable Energy, Sustainability and the Environment,Biotechnology
Cited by
5 articles.
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