Author:
Alam Syed Imteyaz,Bansod Sunita,Singh Lokendra
Abstract
Abstract
Background
Clostridium tetani and Clostridium perfringens are among the medically important clostridial pathogens causing diseases in man and animals. Several homologous open reading frames (ORFs) have been identified in the genomes of the two pathogens by comparative genomic analysis. We tested a likelihood of extensive sharing of common epitopes between homologous proteins of these two medically important pathogens and the possibility of cross-protection using active immunization.
Results
Eight predominant cross-reactive spots were identified by mass spectrometry and had hits in the C. tetani E88 proteome with significant MOWSE scores. Most of the cross-reactive proteins of C. tetani shared 65–78% sequence similarity with their closest homologues in C. perfringens ATCC13124. Electron transfer flavoprotein beta-subunit (CT3) was the most abundant protein (43.3%), followed by methylaspartate ammonia-lyase (36.8%) and 2-phosphoglycerate dehydratase (35.6%). All the proteins were predicted to be cytoplasmic by PSORT protein localization algorithm. Active immunization with C. perfringens whole cells elicited cross-protective immunity against C. tetani infection in a mouse model.
Conclusion
Most of the dominant cross-reactive proteins of C. tetani belonged to the cluster of orthologous group (COG) functional category, either of posttranslational modification, protein turnover, and chaperones (O) or energy production and conversion (C). The homologs of the identified proteins have been shown to play role in pathogenesis in other Gram-positive pathogenic bacteria. Our findings provide basis for the search of potential vaccine candidates with broader coverage, encompassing more than one pathogenic clostridial species.
Publisher
Springer Science and Business Media LLC
Subject
Microbiology (medical),Microbiology
Reference23 articles.
1. Arnon SS: The Clostridia. Edited by: Rood JI, McClane BA, Songer JG, Titbal RW. 1997, San Diego: Academic Press, 95-115.
2. Roper MH, Vandelaer JH, Gasse FL: Maternal and neonatal tetanus. Lancet. 2007, 370: 1947-1959.
3. MacLennan JD: Anaerobic infections of war wounds in the Middle East. Lancet. 1943, ii: 123-126.
4. Rood JI, Cole ST: Molecular genetics and pathogenesis of Clostridium perfringens. Microbiol Rev. 1991, 55: 621-648.
5. Titball RW, Rood JI: Clostridium perfringens wound infection. Molecular Medical Microbiology. Edited by: Sussman M. 2001, United Kingdom: Academic Press, 1875-1904.
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