Author:
Brandfass Christoph,Karlovsky Petr
Abstract
Abstract
Background
Fusarium head blight (FHB) is a disease of cereal crops, which has a severe impact on wheat and barley production worldwide. Apart from reducing the yield and impairing grain quality, FHB leads to contamination of grain with toxic secondary metabolites (mycotoxins), which pose a health risk to humans and livestock. The Fusarium species primarily involved in FHB are F. graminearum and F. culmorum. A key prerequisite for a reduction in the incidence of FHB is an understanding of its epidemiology.
Results
We describe a duplex-PCR-based method for the simultaneous detection of F. culmorum and F. graminearum in plant material. Species-specific PCR products are identified by melting curve analysis performed in a real-time thermocycler in the presence of the fluorescent dye SYBR Green I. In contrast to multiplex real-time PCR assays, the method does not use doubly labeled hybridization probes.
Conclusion
PCR with product differentiation by melting curve analysis offers a cost-effective means of qualitative analysis for the presence of F. culmorum and F. graminearum in plant material. This method is particularly suitable for epidemiological studies involving a large number of samples.
Publisher
Springer Science and Business Media LLC
Subject
Microbiology (medical),Microbiology
Reference23 articles.
1. Parry DW, Jenkinson P, McLeod L: Fusarium ear blight (scab) in small grain cereals – A review. Plant Pathology. 1995, 44: 207-238.
2. Pieters MN, Freijer J, Baars BJ, Fiolet DCM, van Klaveren J, Slob W: Risk assessment of deoxynivalenol in food: Concentration limits, exposure and effects. Mycotoxins and Food Safety. 2002, New York: Kluwer Academic/Plenum Publ, 504: 235-248.
3. Peraica M, Radic B, Lucic A, Pavlovic M: Toxic effects of mycotoxins in humans. Bulletin of the World Health Organization. 1999, 77: 754-766.
4. D'Mello JPF, Placinta CM, Macdonald AMC: Fusarium mycotoxins: a review of global implications for animal health, welfare and productivity. Animal Feed Science and Technology. 1999, 80: 183-205. 10.1016/S0377-8401(99)00059-0.
5. Anonymous: Verordnung über Höchstmengen an Mykotoxinen in Lebensmitteln (Mykotoxin-Höchstmengenverordnung – MHmV). Bundesgesetzblatt. 1999, Bonn: Bundesanzeiger Verlagsges.m.b.H, 1: 1248-1251.
Cited by
63 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献