Abstract
Abstract
Background
Helicobacter pullorum commonly colonized in the gastrointestinal tract of poultry and caused gastroenteritis. This bacterium could be transmitted to humans through contaminated food and caused colitis and hepatitis. Currently, the genetic characteristics of the H. pullorum were not recognized enough. In this study, the genomes of 23 H. pullorum strains from different counties were comparatively analyzed. Among them, H. pullorum 2013BJHL was the first isolated and reported in China.
Results
The genomes of the studied strains were estimated to vary from 1.55 to 2.03 Mb, with a GC content of ~ 34%. 4064 pan genes and 1267 core genes were obtained from the core-pan genome analysis using the Roary pipeline. Core genome SNPs (cg-SNPs) were obtained using Snippy4 software. Two groups were identified with the phylogenetic analysis based on the cg-SNPs. Some adhesion-related, immune regulation, motility-related, antiphagocytosis-related, toxin-related and quorum sensing related genes were identified as virulence factors. APH(3′)-IIIa, APH(2′’)-If, and AAC(6′)-Ie-APH(2′’)-Ia were identified as antibiotic resistance genes among the H. pullorum genomes. cat, SAT-4 and tetO genes were only identified in 2013BJHL, and tet(C) was identified in MIT98-5489. MIC determination revealed that the 2013BJHL showed acquired resistance to ciprofloxacin, nalidixic acid, tetracycline, gentamicin, streptomycin and erythromycin, only sensitive to ampicillin. The antibiotic resistance genetic determinants on the 2013BJHL genome correlate well with observed antimicrobial susceptibility patterns. Two types of VI secretion system (T6SS) were identified in 52.2% (12/23) the studied strains.
Conclusion
In this study, we obtained the genetic characteristics of H. pullorum from different sources in the world. The comprehensive genetic characteristics of H. pullorum were first described. H. pullorum showed highly genetic diversity and two sub-types of T6SSs were first identified in H. pullorum. 2013BJHL was found to be multidrug resistant as it was resistant to at least three different antibiotic classes.
Funder
the Sanming Project of Medicine in Shenzhen
the National Key Program of China
Publisher
Springer Science and Business Media LLC
Subject
Infectious Diseases,Virology,Gastroenterology,Microbiology,Parasitology
Reference25 articles.
1. Behroo S, Javadi A, Rad MG. Helicobacter pullorum prevalence in patients with gastroenteritis in humans and chicken in the province of Ardabil in 2014. Indian J Fundam Appl Life Sci. 2014;5:87–94. https://www.cibtech.org/J-LIFE-SCIENCES/PUBLICATIONS/2015/Vol-5-No-2/13-JLS-012-%20FSHIN-HELICOBACTER.pdf.
2. Bleumink-Pluym NM, van Alphen LB, Bouwman LI, Wösten MM, van Putten J. Identification of a functional type VI secretion system in Campylobacter jejuni conferring capsule polysaccharide sensitive cytotoxicity. PLoS Pathog. 2013;9(5):e1003393. https://doi.org/10.1371/journal.ppat.1003393.
3. Bohr U, Glasbrenner B, Primus A, Zagoura A, Wex T, Malfertheiner P. Identification of enterohepatic Helicobacter species in patients suffering from inflammatory bowel disease. J Clin Microbiol. 2004;42(6):2766–8. https://doi.org/10.1128/jcm.42.6.2766-2768.2004.
4. Borges V, Santos A, Correia C, Saraiva M, Ménard A, Vieira L, et al. Helicobacter pullorum isolated from fresh chicken meat: antibiotic resistance and genomic traits of an emerging foodborne pathogen. Appl Environ Microbiol. 2015;81(23):8155–63. https://doi.org/10.1128/aem.02394-15.
5. Ceelen L, Decostere A, Verschraegen G, Ducatelle R, Haesebrouck F. Prevalence of Helicobacter pullorum among patients with gastrointestinal disease and clinically healthy persons. J Clin Microbiol. 2005;43(6):2984–6. https://doi.org/10.1128/jcm.43.6.2984-2986.2005.
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