Abstract
Abstract
Background
In traditional Malay medicine, Marantodes pumilum (Blume) Kuntze (family Primulaceae) is commonly used by women to treat parturition, flatulence, dysentery, dysmenorrhea, gonorrhea, and bone diseases. Preliminary screening of some Primulaceae species showed that they possess xanthine oxidase inhibitory activity. Thus, this study aimed to investigate the xanthine oxidase inhibitory activity of three varieties of M. pumilum and their phytochemical compounds.
Method
Dichloromethane, methanol, and water extracts of the leaves and roots of M. pumilum var. alata, M. pumilum var. pumila, and M. pumilum var. lanceolata were tested using an in vitro xanthine oxidase inhibitory assay. Bioassay-guided fractionation and isolation were carried out on the most active extract using chromatographic techniques. The structures of the isolated compounds were determined using spectroscopic techniques.
Results
The most active dichloromethane extract of M. pumilum var. pumila leaves (IC50 = 161.6 μg/mL) yielded one new compound, 3,7-dihydroxy-5-methoxy-4,8-dimethyl-isocoumarin (1), and five known compounds, viz. ardisiaquinone A (2), maesanin (3), stigmasterol (4), tetracosane (5), and margaric acid (6). The new compound was found to be the most active xanthine oxidase inhibitor with an IC50 value of 0.66 ± 0.01 μg/mL, which was not significantly different (p > 0.05) from that of the positive control, allopurinol (IC50 = 0.24 ± 0.00 μg/mL).
Conclusion
This study suggests that the new compound 3,7-dihydroxy-5-methoxy-4,8-dimethyl-isocoumarin (1), which was isolated from the dichloromethane extract of M. pumilum var. pumila leaves, could be a potential xanthine oxidase inhibitor.
Funder
Ministry of Agriculture and Agro-Based Industry, Malaysia
Universiti Kebangsaan Malaysia
Publisher
Springer Science and Business Media LLC
Subject
Complementary and alternative medicine
Reference63 articles.
1. The Plant List. Version 1.1. Published on the Internet; http://www.theplantlist.org/ (Accessed 6 April 2020). 2013.
2. Stone BC. New and noteworthy Malesian Myrsinaceae III. On the genus Ardisia Sw. Borneo Proc Acad Nat Sci Philadelphia. 1989;141:263–306.
3. Sunarno B. Revision of the genus Labisia (Myrsinaceae). Blumea. 2005;50(3):579–97.
4. Aladdin NA, Jamal JA, Talip N, Hamsani NAM, Rahman MRA, Sabandar CW, Muhammad K, Husain K, Jalil J. Comparative study of three Marantodes pumilum varieties by microscopy, spectroscopy and chromatography. Rev Bras Farmacogn. 2016;26(1):1–14.
5. Burkill IH. Dictionary of the Economic Products of the Malay Peninsula. Crown Agents of the Colonies, London, England. Vol. 2, 1935;1290.
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