Author:
Toh Seng Chiew,Lihan Samuel,Bunya Scholastica Ramih,Leong Sui Sien
Abstract
Abstract
Background
Cellulitis is a common skin disease encountered in medical emergencies in hospitals. It can be treated using a combination of antibiotics therapy; however, the causative agent Staphylococcus aureus has been reported to develop resistance towards the currently used antibiotics. Therefore, the search for more alternative herbal origin antimicrobial agents is critical. Aim: In this study, maceration and Soxhlet extraction of the whole plant of Cassia alata Linn. (leaves, roots, and stem) were performed using four solvents with different polarities, namely n-hexane, ethyl acetate, ethanol and distilled water. The crude extracts were screened using agar well diffusion, colorimetric broth microdilution, grid culture and bacterial growth curve analysis against Staphylococcus aureus. The phytochemicals in the crude extracts were identified using Gas Chromatography-Mass Spectrometry (GC–MS).
Results
Agar-well diffusion analysis revealed that extraction using ethyl acetate showed the largest inhibition zone with an average diameter of 15.30 mm (root Soxhlet extract) followed by 14.70 mm (leaf Soxhlet extract) and 13.70 mm (root maceration extract). The lowest minimum inhibitory and minimum bactericidal concentration in root Soxhlet extract using ethyl acetate was 0.313 and 0.625 µg µL−1, respectively. Our study proved that crude extract of the plant suppressed the growth of S. aureus as evidenced from a significant regression extension (p < 0.06, p = 0.00003) of lag phase for 6 h after the treatment with increased concentration. Based on the GC–MS analysis, 88 phytochemicals consist of fatty acids, esters, alkanes, phenols, fatty alcohols, sesquiterpenoids and macrocycle that possibly contributed to the antimicrobial properties were identified, 32 of which were previously characterized for their antimicrobial, antioxidant, and anti-inflammatory activities.
Conclusion
Ethyl acetate crude extract was better than the other investigated solvents. The root and stem of C. alata showed significant antimicrobial efficacy against S. aureus in this study. The remaining 56 out of 88 phytochemicals of the plant should be intensively studied for more medicinal uses.
Funder
Tun Zaidi Chair
Putra Grant, Universiti Putra Malaysia
Publisher
Springer Science and Business Media LLC
Subject
Complementary and alternative medicine
Reference105 articles.
1. Swartz MN. Cellulitis. N Engl J Med. 2004;350(9):904–12. https://doi.org/10.1056/NEJMcp031807.
2. Joseph J, Abraham S, Soman A, Mathew LK, Ganga SV, Vijayan V. Cellulitis: a bacterial skin infection, their causes, diagnosis and treatment. World J Pharm Pharm Sci. 2014;3(7):308–26.
3. US FDA. Guidance for industry. Acute bacterial skin and skin structure infections: Developing drugs for treatment. Silver Spring (MD). USA: US Department of Health and Human Services, Food and Drug Administration, Center for Drug Evaluation and Research (CDER); 2013.
4. Gardner E. South West Essex Community Services (SWECS) adult primary care cellulitis guideline. 3rd ed. UK: National Health Services; 2013.
5. Björnsdóttir S, Gottfredsson M, Thórisdóttir AS, Gunnarsson GB, Rıkardsdóttir H, Kristjánsson M, Hilmarsdóttir I. Risk factors for acute cellulitis of the lower limb: a prospective case-control study. Clin Infect Dis. 2005;41(10):1416–22. https://doi.org/10.1086/497127.