Abstract
Abstract
Background
Breast cancer is the most common malignancy in women, and medicinal plants can prevent and play an inhibitory role for cancer. This study aims to evaluate the anticancer effect of colchicum autumnale L. Corm on breast cancer cell models.
Methods
In this study, the alkaloid-rich extract was prepared using the percolation method and with methanol/water solvent (70:30). HFF2 normal cell line and MCF-7 breast cancer cell line were cultured in microplates (96 wells). Then cells were treated with concentrations of 62.5 to 2000 ng/ml of extract and concentrations of 62 to 1000 ng/ml of doxorubicin at regular intervals of 48 and 72 h, and the percentage of cell growth inhibition was calculated. Cytotoxicity of drugs was measured by the MTT assay method. IC50 values were calculated by Calcusyn software. Also, the P-value of < 0.05 was considered significant.
Results
Alkaloid-rich extract of Colchicum autumnale plant inhibited breast cancer cell growth (MCF-7). The IC50 parameter showed more cytotoxic effects of Colchicum autumnale plant extract on the MCF-7 cancer cell line than HFF2 normal cell line for 48 and 72 h. In addition, with higher concentrations of the extract, cytotoxicity, and growth inhibitory effect increased significantly and in comparison to the doxorubicin was almost the same as cytotoxic.
Conclusion
This research provides a novel view into the development of new drugs for the treatment of cancer diseases. Colchicum autumnale plant extract had a significant cytotoxic effect like Doxorubicin drug on breast cancer cell line (MCF-7), which can alternatively treat and prevent breast cancer.
Publisher
Springer Science and Business Media LLC
Subject
Complementary and alternative medicine
Reference35 articles.
1. Tahergorabi Z, Moodi M, Mesbahzadeh B. Breast Cancer: A preventable disease. J Birjand Univ Med Sci. 2014;21(2):41–126.
2. Ali S, Noreen A, Qamar A, Zafar I, Ain Q, Nafidi H-A, et al. Amomum subulatum: A treasure trove of anticancer compounds targeting TP53 protein using in vitro and in silico techniques. Front Chem. 2023;11:1174363. https://doi.org/10.3389/fchem.2023.1174363.
3. Organization WH. Cancer. 2022. Available from: https://www.who.int/news-room/fact-sheets/detail/cancer.
4. Abachizadeh K, Keramatinia AA. Anticipating Cancer Rates of Iran in 2025. Community Health. 2016;3(1):66–73.
5. Tabarestani S, Noori Daloii MR. Molecular genetics, diagnosis and treatment of breast cancer: review article. Journal of Sabzevar University of Medical Sciences. 2010;17(2):74–87. https://jsums.medsab.ac.ir/article_62.html?lang=en.
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献