Unraveling the treatment effects of huanglian jiedu decoction on drug-induced liver injury based on network pharmacology, molecular docking and experimental validation-Reference-Cited by-同舟云学术

Unraveling the treatment effects of huanglian jiedu decoction on drug-induced liver injury based on network pharmacology, molecular docking and experimental validation

Published:2024-06-07 Issue:1 Volume:24 Page:
ISSN:2662-7671
Container-title:BMC Complementary Medicine and Therapies
language:en
Short-container-title:BMC Complement Med Ther

Author:

Xie Yaochen,Gong Shuchen,Wang Lingkun,Yang Zhaoxu,Yang Chen,Li Guilin,Zha Huiyan,Lv Shuying,Xiao Boneng,Chen Xiaoyu,Di Zhenning,He Qiaojun,Wang Jincheng,Weng Qinjie

Abstract

AbstractHuanglian Jiedu Decoction (HJD) is a well-known Traditional Chinese Medicine formula that has been used for liver protection in thousands of years. However, the therapeutic effects and mechanisms of HJD in treating drug-induced liver injury (DILI) remain unknown. In this study, a total of 26 genes related to both HJD and DILI were identified, which are corresponding to a total of 41 potential active compounds in HJD. KEGG analysis revealed that Tryptophan metabolism pathway is particularly important. The overlapped genes from KEGG and GO analysis indicated the significance of CYP1A1, CYP1A2, and CYP1B1. Experimental results confirmed that HJD has a protective effect on DILI through Tryptophan metabolism pathway. In addition, the active ingredients Corymbosin, and Moslosooflavone were found to have relative strong intensity in UPLC-Q-TOF-MS/MS analysis, showing interactions with CYP1A1, CYP1A2, and CYP1B1 through molecule docking. These findings could provide insights into the treatment effects of HJD on DILI.

Funder

Fundamental Research Funds for the Central Universities

Zhejiang Provincial Natural Science Foundation

Publisher

Springer Science and Business Media LLC

Link

https://link.springer.com/content/pdf/10.1186/s12906-024-04517-y.pdf

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