Human endogenous retrovirus K in the respiratory tract is associated with COVID-19 physiopathology
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Published:2022-04-22
Issue:1
Volume:10
Page:
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ISSN:2049-2618
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Container-title:Microbiome
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language:en
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Short-container-title:Microbiome
Author:
Temerozo Jairo R., Fintelman-Rodrigues Natalia, dos Santos Monique Cristina, Hottz Eugenio D., Sacramento Carolina Q., de Paula Dias da Silva Aline, Mandacaru Samuel Coelho, dos Santos Moraes Emilly Caroline, Trugilho Monique R. O., Gesto João S. M., Ferreira Marcelo Alves, Saraiva Felipe Betoni, Palhinha Lohanna, Martins-Gonçalves Remy, Azevedo-Quintanilha Isaclaudia Gomes, Abrantes Juliana L., Righy Cássia, Kurtz Pedro, Jiang Hui, Tan Hongdong, Morel Carlos, Bou-Habib Dumith Chequer, Bozza Fernando A., Bozza Patrícia T., Souza Thiago Moreno L.ORCID
Abstract
Abstract
Background
Critically ill 2019 coronavirus disease (COVID-19) patients under invasive mechanical ventilation (IMV) are 10 to 40 times more likely to die than the general population. Although progression from mild to severe COVID-19 has been associated with hypoxia, uncontrolled inflammation, and coagulopathy, the mechanisms involved in the progression to severity are poorly understood.
Methods
The virome of tracheal aspirates (TA) from 25 COVID-19 patients under IMV was assessed through unbiased RNA sequencing (RNA-seq), and correlation analyses were conducted using available clinical data. Unbiased sequences from nasopharyngeal swabs (NS) from mild cases and TA from non-COVID patients were included in our study for further comparisons.
Results
We found higher levels and differential expression of human endogenous retrovirus K (HERV-K) genes in TA from critically ill and deceased patients when comparing nasopharyngeal swabs from mild cases to TA from non-COVID patients. In critically ill patients, higher HERV-K levels were associated with early mortality (within 14 days of diagnosis) in the intensive care unit. Increased HERV-K expression in deceased patients was associated with IL-17-related inflammation, monocyte activation, and an increased consumption of clotting/fibrinolysis factors. Moreover, increased HERV-K expression was detected in human primary monocytes from healthy donors after experimental SARS-CoV-2 infection in vitro.
Conclusion
Our data implicate the levels of HERV-K transcripts in the physiopathology of COVID-19 in the respiratory tract of patients under invasive mechanical ventilation.
Funder
Conselho Nacional de Desenvolvimento Científico e Tecnológico Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro
Publisher
Springer Science and Business Media LLC
Subject
Microbiology (medical),Microbiology
Reference75 articles.
1. Dong E, Du H, Gardner L. An interactive web-based dashboard to track COVID-19 in real time. Lancet Infect Dis. 2020;20:533–4. 2. Benefield AE, Skrip LA, Clement A, Althouse RA, Chang S, Althouse BM. SARS-CoV-2 viral load peaks prior to symptom onset: a systematic review and individual-pooled analysis of coronavirus viral load from 66 studies. medRxiv. 2020:2020.09.28.20202028. 3. Henry BM, Aggarwal G, Wong J, Benoit S, Vikse J, Plebani M, et al. Lactate dehydrogenase levels predict coronavirus disease 2019 (COVID-19) severity and mortality: a pooled analysis. Am J Emerg Med. 2020;38:1722–6. 4. Laing AG, Lorenc A, Del Barrio IDM, Das A, Fish M, Monin L, et al. A dynamic COVID-19 immune signature includes associations with poor prognosis. Nat Med. 2020;26:1623–35. 5. Dorward DA, Russell CD, Um IH, Elshani M, Armstrong SD, Penrice-Randal R, et al. Tissue-specific immunopathology in fatal COVID-19. Am J Respir Crit Care Med. 2021;203:192–201.
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