Author:
Sekete Malota,Ma Duanzheng,Wang Bo,Wang Hexiang,Ng Tzibun
Abstract
Abstract
A 45-kDa ribonuclease (RNase) was purified from dried fruiting bodies of the wild mushroom Amanita hemibapha. It was adsorbed on DEAE-cellulose, S-sepharose, and finally purified on Superdex 75. The RNase exhibited maximal RNase activity at pH 5 and in a temperature range between 60-70°C. It demonstrated no ribonucleolytic activity toward four polyhomoribonucleotides. The amino acid sequence analysis (GDDETFWEHEWAK) showed this RNase was a ribonuclease T2-like RNase. It exhibited strong inhibitory activity against HIV-1 reverse transcriptase (HIV-1 RT) with an IC50 of 17 μM.
Publisher
Springer Science and Business Media LLC
Reference32 articles.
1. Collins RA, Ng TB, Fong WP, Wan CC, Yeung HW: A comparison of human immunodeficiency virus type 1 inhibition by partially purified aqueous extracts of Chinese medicinal herbs. Life Sci 1997, 60: L345-L351.
2. Fang EF, Ng TB: Ribonucleases of different origins with a wide spectrum of medicinal applications. Biochimica et Biophysica Acta 2010, 1815: 65-74.
3. Guan GP, Wang HX, Ng TB: A novel ribonuclease with antiproliferative activity from fresh fruiting bodies of the edible mushroom Hypsizigus marmoreus. Biochim Biophys Acta 2007, 1770: 1593-1597. 10.1016/j.bbagen.2007.07.014
4. Hofsteenge J, Matthies R, Stone SR: Primary structure of a ribonuclease from porcine liver, a new member of the ribonuclease superfamily. Biochemistry 1989, 28: 9806-9813. 10.1021/bi00451a040
5. Iwama M, Sanda A, Ohgi K, Hofsteenge J, Irie M: Purification and primary structure of a porcine kidney non-secretory ribonuclease. Biosci Biotechnol Biochem 1993, 57: 2133-2138. 10.1271/bbb.57.2133
Cited by
6 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献