Author:
Wang Tianqi,Liu Ziwen,Wang Xinrui,Li Yuhua,AKHTAR FAHEEM,Li Mengmeng,Zhang Zhenwei,Zhan Yandong,Shi Xiaoyuan,Ren Wei,Huang Bingjian,Wang Changfa,Chai Wenqiong
Abstract
Abstract
Background
Previous studies have shown that the protein kinase cGMP-dependent 2 (PRKG2) gene is associated with dwarfism in humans, dogo Argentines, and Angus cattle, as well as with height and osteoblastogenesis in humans. Therefore, the PRKG2 gene was used as the target gene to explore whether this gene is associated with several thoracolumbar vertebrae and carcass traits in Dezhou donkeys.
Results
In this study, fifteen SNPs were identified by targeted sequencing, all of which were located in introns of the PRKG2 gene. Association analysis illustrated that the g.162153251 G > A, g.162156524 C > T, g.162158453 C > T and, g.162163775 T > G were significantly different from carcass weight. g.162166224 G > A, g.162166654 T > A, g.162167165 C > A, g.162167314 A > C and, g.162172653 G > C were significantly associated with the number of thoracic vertebrae. g.162140112 A > G was significantly associated with the number and the length of lumbar vertebrae, and g.162163775 T > G was significantly associated with the total number of thoracolumbar vertebrae.
Conclusion
Overall, the results of this study suggest that PRKG2 gene polymorphism can be used as a molecular marker to breed high-quality Dezhou donkeys.
Publisher
Springer Science and Business Media LLC
Subject
Health Informatics,Genetics
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