Author:
Zhu Fei,Lu Jing,Sun Ke,Deng Cao,Xu Yu
Abstract
Abstract
Background
Indotyphlops braminus, the only known triploid parthenogenetic snake, is a compelling species for revealing the mechanism of polyploid emergence in vertebrates.
Methods
In this study, we applied PacBio isoform sequencing technology to generate the first full-length transcriptome of I. braminus, aiming to improve the understanding of the molecular characteristics of this species.
Results
A total of 51,849 nonredundant full-length transcript assemblies (with an N50 length of 2980 bp) from I. braminus were generated and fully annotated using various gene function databases. Our analysis provides preliminary evidence supporting a recent genome duplication event in I. braminus. Phylogenetic analysis indicated that the divergence of I. braminus subgenomes occurred approximately 11.5 ~ 15 million years ago (Mya). The full-length transcript resource generated as part of this research will facilitate transcriptome analysis and genomic evolution studies in the future.
Funder
National Natural Science Foundation of China and the Karst Science Research Center of Guizhou Province
New seedling plans from Guizhou Normal University
Publisher
Springer Science and Business Media LLC
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