Author:
Liu Zhuohao,Zhou Jiayi,Yang Xinzhi,Liu Yuchen,Zou Chang,Lv Wen,Chen Cheng,Cheng Kenneth King-yip,Chen Tao,Chang Lung-Ji,Wu Dinglan,Mao Jie
Abstract
Abstract
Background
This study aimed to validate whether infusion of GD2-specific fourth-generation safety-designed chimeric antigen receptor (4SCAR)-T cells is safe and whether CAR-T cells exert anti-glioblastoma (GBM) activity.
Methods
A total of eight patients with GD2-positive GBM were enrolled and infused with autologous GD2-specific 4SCAR-T cells, either through intravenous administration alone or intravenous combined with intracavitary administration.
Results
4SCAR-T cells expanded for 1–3 weeks and persisted at a low frequency in peripheral blood. Of the eight evaluable patients, four showed a partial response for 3 to 24 months, three had progressive disease for 6 to 23 months, and one had stable disease for 4 months after infusion. For the entire cohort, the median overall survival was 10 months from the infusion. GD2 antigen loss and infiltrated T cells were observed in the tumor resected after infusion.
Conclusion
Both single and combined infusions of GD2-specific 4SCAR-T cells in targeting GBM were safe and well tolerated, with no severe adverse events. In addition, GD2-specific 4SCAR-T cells partially mediate antigen loss and activate immune responses in the tumor microenvironment. Validation of our findings in a larger prospective trial is warranted.
Trial registration
ClinicalTrials.gov Identifier: NCT03170141. Registered 30 May 2017.
Funder
National Natural Science Foundation of China
Shenzhen Science and Technology Innovation Program
Publisher
Springer Science and Business Media LLC
Subject
Cancer Research,Oncology,Molecular Medicine
Cited by
46 articles.
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