Author:
Qiao Kaikai,Zeng Qingtao,Lv Jiaoyan,Chen Lingling,Hao Juxin,Wang Ding,Ma Qifeng,Fan Shuli
Abstract
Abstract
Background
Neutral/alkaline invertases (N/AINVs) play a crucial role in plant growth, development, and stress response, by irreversibly hydrolyzing sucrose into glucose and fructose. However, research on cotton in this area is limited. This study aims to investigate GhN/AINV23, a neutral/alkaline invertase in cotton, including its characteristics and biological functions.
Results
In our study, we analyzed the sequence information, three-dimensional (3D) model, phylogenetic tree, and cis-elements of GhN/AINV23. The localization of GhN/AINV23 was determined to be in the cytoplasm and cell membrane. Quantitative real-time polymerase chain reaction (qRT-PCR) results showed that GhN/AINV23 expression was induced by abscisic acid (ABA), exogenous sucrose and low exogenous glucose, and inhibited by high exogenous glucose. In Arabidopsis, overexpression of GhN/AINV23 promoted vegetative phase change, root development, and drought tolerance. Additionally, the virus-induced gene silencing (VIGS) assay indicated that the inhibition of GhN/AINV23 expression made cotton more susceptible to drought stress, suggesting that GhN/AINV23 positively regulates plant drought tolerance.
Conclusion
Our research indicates that GhN/AINV23 plays a significant role in plant vegetative phase change, root development, and drought response. These findings provide a valuable foundation for utilizing GhN/AINV23 to improve cotton yield.
Funder
the “Tianshan Talents” Youth Science and Technology Outstanding Talent Project - Grassroots Science and Technology Backbone Talent,
Natural Science Foundation of Xinjiang Uygur Autonomous Region
the Project of Sanya Yazhou Bay Science and Technology City
Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences
Publisher
Springer Science and Business Media LLC