Development and evaluation of a recombinase polymerase amplification assay for rapid detection of strawberry red stele pathogen

Author:

Munawar Mustafa AhmadORCID,Toljamo Anna,Martin Frank,Oksanen Elina,Kokko Harri

Abstract

AbstractPhytophthora fragariae causes drastic damage in strawberry crops. P. fragariae infects strawberry roots and causes red stele root rot. Although P. fragariae is a quarantine organism, its spread in Finland continues as more and more fields contract the disease. The spread can be halted through developing rapid and reliable detection assays. We have developed a rapid recombinase polymerase amplification (RPA) assay for P. fragariae targeting the Phytophthora mitochondrial DNA intergenic atp9-nad9 marker. The assay is DNA-extraction free and capable of detecting as low as 10 fg of P. fragariae genomic DNA. We found the assay reliable for diagnosing field plants when samples are adequately collected. We also applied the RPA assay to the detection of the pathogen in the soil through coupling the assay with baiting with the host plant. The results suggest that if only a small number of samples are analysed, the baiting results will not be reliable.

Funder

European Agricultural Fund for Rural Development (EAFRD), Pohjois-Savon ELY-Keskus

Publisher

Springer Science and Business Media LLC

Subject

General Medicine

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