Author:
Pereira Hugo,Barreira Luísa,Mozes André,Florindo Cláudia,Polo Cristina,Duarte Catarina V,Custódio Luísa,Varela João
Abstract
Abstract
We describe a new selection method based on BODIPY (4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene) staining, fluorescence activated cell sorting (FACS) and microplate-based isolation of lipid-rich microalgae from an environmental sample. Our results show that direct sorting onto solid medium upon FACS can save about 3 weeks during the scale-up process as compared with the growth of the same cultures in liquid medium. This approach enabled us to isolate a biodiverse collection of several axenic and unialgal cultures of different phyla.
Publisher
Springer Science and Business Media LLC
Subject
Management, Monitoring, Policy and Law,General Energy,Renewable Energy, Sustainability and the Environment,Applied Microbiology and Biotechnology,Biotechnology
Cited by
126 articles.
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