Author:
Nejat Farhad,Jadidi Khosrow,Amoli Fahimeh Asadi,Bagheri Shiva,Aghamollaei Hossein,Nejat Mohammad-Amin,Nabavi Nazanin-Sadat,Eghtedari Shima
Abstract
Abstract
Background
Plasma medicine is an innovative research field focused on the application of atmospheric-pressure low-temperature plasmas (ALTP) for therapeutic purposes. Considering the potentials of plasma in ophthalmology, in this study, we evaluated the safety of plasma on the conjunctival tissue in animal models for 6 months.
Methods
Twelve adult male New Zealand albino rabbits were divided into four groups. The right eye of each rabbit was chosen for the test and the left eye was considered as the control. Experiments were performed using the Plexr device (GMV, Rocca Priora, RM, Italy). Four plasma spots were applied on the superior part of the conjunctiva (from 10 to 2 o’clock positions) using the continuous mode and a low power level (white handpiece) of the Plexr. For evaluation of the plasma safety, the histopathological changes were assessed 1 week (A), 1 month (B), 3 months (C), and 6 months (D) after the intervention.
Results
According to the histopathological findings, a mild decrease in blood vessels and severe stromal edema, as well as a superficial epithelium loss, were observed in group A. No chronic inflammation, scar tissue, deposition, and hemorrhage were found in group B. Epithelialization was confirmed by the histological examinations after 1 month. There was no evidence of atypia or dysplasia after 3 and 6 months.
Conclusion
In conclusion, there were no persistent histopathological changes on conjunctival tissue after plasma exposure. Then, plasma can be considered as a minimally invasive alternative method for treating some ocular surface disorders.
Publisher
Springer Science and Business Media LLC
Subject
Ophthalmology,General Medicine
Reference28 articles.
1. Von Woedtke T, Reuter S, Masur K, Weltmann K-D. Plasmas for medicine. Phys Rep. 2013;530(4):291–320. https://doi.org/10.1016/j.physrep.2013.05.005.
2. Gerling T, Weltmann KD. Einführung in Atmosphärendruck-Plasmaquellen für plasmamedizinische Anwendungen. Plasmamedizin: Springer; 2016. p. 3–15.
3. Conrads H, Schmidt M. Plasma generation and plasma sources. Plasma Sources Sci Technol. 2000;9(4):441–54. https://doi.org/10.1088/0963-0252/9/4/301.
4. Chaudhuri M, Ivlev AV, Khrapak SA, Thomas HM, Morfill GE. Complex plasma—the plasma state of soft matter. Soft Matter. 2011;7(4):1287–98. https://doi.org/10.1039/C0SM00813C.
5. Laroussi M, Keidar M. Plasma processes and polymers special issue on: plasma and cancer; 2014.
Cited by
3 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献